Shewanella irciniae sp. nov., a novel member of the family Shewanellaceae, isolated from the marine sponge Ircinia dendroides in the Bay of Villefranche, Mediterranean Sea

On On Lee, Stanley C K Lau, Mandy M Y Tsoi, Xiancui Li, Ioulia Plakhotnikova, Sergey Dobretsov, Madeline C S Wu, Po Keung Wong, Markus Weinbauer, Pei Yuan Qian

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Strain UST040317-058T, comprising non-pigmented, rod-shaped, facultatively anaerobic, Gram-negative cells that are motile by means of single polar flagella, was isolated from the surface of a marine sponge (Ircinia dendroides) collected from the Mediterranean Sea. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a separate cluster with the recognized bacterium Shewanella algae IAM 14159T, with which it showed a sequence similarity of 95.0%. The sequence similarity between strain UST040317-058T and its other (six) closest relatives ranged from 91.6 to 93.8%. Strain UST040317-058T showed oxidase, catalase and gelatinase activities. The typical respiratory quinones for shewanellas, menaquinone MK-7 and ubiquinones Q-7 and Q-8, were also detected. The predominant fatty acids in strain UST040317-058T were i15:0, 16:0, 17:1ω8c and summed feature 3 (comprising i15:0 2-OH and/or 16:1ω7c), altogether representing 56.9% of the total. The DNA G + C content was 39.9 mol%. The strain could be differentiated from other Shewanella species by its inability to reduce nitrate or produce H2S and by 10-22 additional phenotypic characteristics. On the basis of the phylogenetic and phenotypic data presented in this study, strain UST040317-058T represents a novel species in the genus Shewanella, for which the name Shewanella irciniae sp. nov. is proposed. The type strain is UST040317-058T (=JCM 13528T = NRRL B-41466T).

Original languageEnglish
Pages (from-to)2871-2877
Number of pages7
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume56
Issue number12
DOIs
Publication statusPublished - Dec 2006

Fingerprint

Shewanella
Mediterranean Sea
Porifera
sponge
phylogenetics
new species
Gram-Negative Facultatively Anaerobic Rods
fatty acid
alga
nitrate
DNA
Gelatinases
Quinones
bacterium
Flagella
gene
Base Composition
rRNA Genes
Nitrates
Catalase

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Shewanella irciniae sp. nov., a novel member of the family Shewanellaceae, isolated from the marine sponge Ircinia dendroides in the Bay of Villefranche, Mediterranean Sea. / Lee, On On; Lau, Stanley C K; Tsoi, Mandy M Y; Li, Xiancui; Plakhotnikova, Ioulia; Dobretsov, Sergey; Wu, Madeline C S; Wong, Po Keung; Weinbauer, Markus; Qian, Pei Yuan.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 56, No. 12, 12.2006, p. 2871-2877.

Research output: Contribution to journalArticle

Lee, On On ; Lau, Stanley C K ; Tsoi, Mandy M Y ; Li, Xiancui ; Plakhotnikova, Ioulia ; Dobretsov, Sergey ; Wu, Madeline C S ; Wong, Po Keung ; Weinbauer, Markus ; Qian, Pei Yuan. / Shewanella irciniae sp. nov., a novel member of the family Shewanellaceae, isolated from the marine sponge Ircinia dendroides in the Bay of Villefranche, Mediterranean Sea. In: International Journal of Systematic and Evolutionary Microbiology. 2006 ; Vol. 56, No. 12. pp. 2871-2877.
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abstract = "Strain UST040317-058T, comprising non-pigmented, rod-shaped, facultatively anaerobic, Gram-negative cells that are motile by means of single polar flagella, was isolated from the surface of a marine sponge (Ircinia dendroides) collected from the Mediterranean Sea. Comparative 16S rRNA gene sequence-based phylogenetic analysis placed the strain in a separate cluster with the recognized bacterium Shewanella algae IAM 14159T, with which it showed a sequence similarity of 95.0{\%}. The sequence similarity between strain UST040317-058T and its other (six) closest relatives ranged from 91.6 to 93.8{\%}. Strain UST040317-058T showed oxidase, catalase and gelatinase activities. The typical respiratory quinones for shewanellas, menaquinone MK-7 and ubiquinones Q-7 and Q-8, were also detected. The predominant fatty acids in strain UST040317-058T were i15:0, 16:0, 17:1ω8c and summed feature 3 (comprising i15:0 2-OH and/or 16:1ω7c), altogether representing 56.9{\%} of the total. The DNA G + C content was 39.9 mol{\%}. The strain could be differentiated from other Shewanella species by its inability to reduce nitrate or produce H2S and by 10-22 additional phenotypic characteristics. On the basis of the phylogenetic and phenotypic data presented in this study, strain UST040317-058T represents a novel species in the genus Shewanella, for which the name Shewanella irciniae sp. nov. is proposed. The type strain is UST040317-058T (=JCM 13528T = NRRL B-41466T).",
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AU - Lau, Stanley C K

AU - Tsoi, Mandy M Y

AU - Li, Xiancui

AU - Plakhotnikova, Ioulia

AU - Dobretsov, Sergey

AU - Wu, Madeline C S

AU - Wong, Po Keung

AU - Weinbauer, Markus

AU - Qian, Pei Yuan

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