Selective anti-cancer activity against melanoma cells using 3-O-acetyl-β-boswellic acid-loaded 3D-Printed scaffold

Fatemeh Jamshidi-adegani; Saeid Vakilian; Sulaiman Al-Hashmi; Juhaina Al-Kindi; Najeeb Ur Rehman; Yaqeen Al-Sinani; Shokoofeh Ghaemi; Khurshid Alam; Muhammad U. Anwar; Rene Csuk; Ahmed Al-Harrasi

doi:10.1080/14786419.2022.2116024

Selective anti-cancer activity against melanoma cells using 3-O-acetyl-β-boswellic acid-loaded 3D-Printed scaffold

Fatemeh Jamshidi-adegani, Saeid Vakilian, Sulaiman Al-Hashmi^*, Juhaina Al-Kindi, Najeeb Ur Rehman, Yaqeen Al-Sinani, Shokoofeh Ghaemi, Khurshid Alam, Muhammad U. Anwar, Rene Csuk, Ahmed Al-Harrasi^*

^*Corresponding author for this work

Mechanical and Industrial Engineering

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

This study aimed to develop a local 3 D-printed bioactive graft using poly-caprolacton (PCL) as a drug carrier and 3-O-acetyl-β-boswellic acid (β-ABA) as an anticancer compound. β-ABA-loaded 3 D-printed scaffold was fabricated and physically characterized. The results indicated more desirable mechanical and physical properties of the β-ABA-loaded PCL mat in comparison with the PCL scaffold. Following sustained release of β-ABA, the β-ABA-loaded PCL scaffold revealed selective cytotoxic activity against melanoma cells, while the PCL + ABA with the bolus delivery of β-ABA was toxic against fibroblast cells. Followed by the induction of apoptosis in melanoma cells at the gene level, the result of the western blot showed that the β-ABA-loaded scaffold significantly up-regulated P53 and down-regulated BCL2, with an increment in the ratio of Bax/BCL2. The selective anti-cancer properties of β-ABA-loaded 3 D printed scaffold against melanoma cells indicated that this scaffold could be potentially used as a bioactive graft to improve the melanoma treatment.

Original language	English
Pages (from-to)	2049-2054
Number of pages	6
Journal	Natural Product Research
Volume	37
Issue number	12
DOIs	https://doi.org/10.1080/14786419.2022.2116024
Publication status	Published - Jun 2023

Keywords

3D-printing
BCL2
boswellic acid
melanoma
p53
skin tissue engineering
sustained delivery
Humans
Melanoma/drug therapy
Proto-Oncogene Proteins c-bcl-2
Printing, Three-Dimensional

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Plant Science
Organic Chemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1080/14786419.2022.2116024

Cite this

Jamshidi-adegani, F., Vakilian, S., Al-Hashmi, S., Al-Kindi, J., Rehman, N. U., Al-Sinani, Y., Ghaemi, S., Alam, K., Anwar, M. U., Csuk, R., & Al-Harrasi, A. (2023). Selective anti-cancer activity against melanoma cells using 3-O-acetyl-β-boswellic acid-loaded 3D-Printed scaffold. Natural Product Research, 37(12), 2049-2054. https://doi.org/10.1080/14786419.2022.2116024

Jamshidi-adegani, F, Vakilian, S, Al-Hashmi, S, Al-Kindi, J, Rehman, NU, Al-Sinani, Y, Ghaemi, S, Alam, K, Anwar, MU, Csuk, R & Al-Harrasi, A 2023, 'Selective anti-cancer activity against melanoma cells using 3-O-acetyl-β-boswellic acid-loaded 3D-Printed scaffold', Natural Product Research, vol. 37, no. 12, pp. 2049-2054. https://doi.org/10.1080/14786419.2022.2116024

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abstract = "This study aimed to develop a local 3 D-printed bioactive graft using poly-caprolacton (PCL) as a drug carrier and 3-O-acetyl-β-boswellic acid (β-ABA) as an anticancer compound. β-ABA-loaded 3 D-printed scaffold was fabricated and physically characterized. The results indicated more desirable mechanical and physical properties of the β-ABA-loaded PCL mat in comparison with the PCL scaffold. Following sustained release of β-ABA, the β-ABA-loaded PCL scaffold revealed selective cytotoxic activity against melanoma cells, while the PCL + ABA with the bolus delivery of β-ABA was toxic against fibroblast cells. Followed by the induction of apoptosis in melanoma cells at the gene level, the result of the western blot showed that the β-ABA-loaded scaffold significantly up-regulated P53 and down-regulated BCL2, with an increment in the ratio of Bax/BCL2. The selective anti-cancer properties of β-ABA-loaded 3 D printed scaffold against melanoma cells indicated that this scaffold could be potentially used as a bioactive graft to improve the melanoma treatment.",

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AU - Jamshidi-adegani, Fatemeh

AU - Vakilian, Saeid

AU - Al-Hashmi, Sulaiman

AU - Al-Kindi, Juhaina

AU - Rehman, Najeeb Ur

AU - Al-Sinani, Yaqeen

AU - Ghaemi, Shokoofeh

AU - Alam, Khurshid

AU - Anwar, Muhammad U.

AU - Csuk, Rene

AU - Al-Harrasi, Ahmed

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AB - This study aimed to develop a local 3 D-printed bioactive graft using poly-caprolacton (PCL) as a drug carrier and 3-O-acetyl-β-boswellic acid (β-ABA) as an anticancer compound. β-ABA-loaded 3 D-printed scaffold was fabricated and physically characterized. The results indicated more desirable mechanical and physical properties of the β-ABA-loaded PCL mat in comparison with the PCL scaffold. Following sustained release of β-ABA, the β-ABA-loaded PCL scaffold revealed selective cytotoxic activity against melanoma cells, while the PCL + ABA with the bolus delivery of β-ABA was toxic against fibroblast cells. Followed by the induction of apoptosis in melanoma cells at the gene level, the result of the western blot showed that the β-ABA-loaded scaffold significantly up-regulated P53 and down-regulated BCL2, with an increment in the ratio of Bax/BCL2. The selective anti-cancer properties of β-ABA-loaded 3 D printed scaffold against melanoma cells indicated that this scaffold could be potentially used as a bioactive graft to improve the melanoma treatment.

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KW - Proto-Oncogene Proteins c-bcl-2

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Selective anti-cancer activity against melanoma cells using 3-O-acetyl-β-boswellic acid-loaded 3D-Printed scaffold

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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