Salt stress alters DNA methylation levels in alfalfa (Medicago spp)

A. Al-Lawati, S. Al-Bahry, R. Victor, A. H. Al-Lawati, M. W. Yaish

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Modification of DNA methylation status is one of the mechanisms used by plants to adjust gene expression at both the transcriptional and posttranscriptional levels when plants are exposed to suboptimal conditions. Under abiotic stress, different cultivars often show heritable phenotypic variation accompanied by epigenetic polymorphisms at the DNA methylation level. This variation may provide the raw materials for plant breeding programs that aim to enhance abiotic stress tolerance, including salt tolerance. In this study, methylation-sensitive amplified polymorphism (MSAP) analysis was used to assess cytosine methylation levels in alfalfa (Medicago spp) roots exposed to increasing NaCl concentrations (0.0, 8.0, 12.0, and 20.0 dS/m). Eleven indigenous landraces were analyzed, in addition to a salt-tolerant cultivar that was used as a control. There was a slight increase in DNA methylation upon exposure to high levels of soil salinity. Phylogenetic analysis using MSAP showed epigenetic variation within and between the alfalfa landraces when exposed to saline conditions. Based on MSAP and enzyme-linked immunosorbent assay results, we found that salinity increased global DNA methylation status, particularly in plants exposed to the highest level of salinity (20 dS/m). Quantitative reverse transcription-polymerase chain reaction indicated that this might be mediated by the overexpression of methyltransferase homolog genes after exposure to saline conditions. DNA demethylation using 5-azacytidine reduced seedling lengths and dry and fresh weights, indicating a possible decrease in salinity tolerance. These results suggest that salinity affects DNA methylation flexibility.

Original languageEnglish
Article number15018299
JournalGenetics and Molecular Research
Volume15
Issue number1
DOIs
Publication statusPublished - Feb 26 2016

Fingerprint

Medicago
Medicago sativa
Salinity
DNA Methylation
Salts
Methylation
Epigenomics
Salt-Tolerance
Azacitidine
Cytosine
Methyltransferases
Seedlings
Reverse Transcription
Soil
Enzyme-Linked Immunosorbent Assay
Gene Expression
Weights and Measures
Polymerase Chain Reaction
DNA
Genes

Keywords

  • Medicago
  • Methylation
  • MSAP
  • Salinity

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Salt stress alters DNA methylation levels in alfalfa (Medicago spp). / Al-Lawati, A.; Al-Bahry, S.; Victor, R.; Al-Lawati, A. H.; Yaish, M. W.

In: Genetics and Molecular Research, Vol. 15, No. 1, 15018299, 26.02.2016.

Research output: Contribution to journalArticle

@article{bc3f1fc80a01410ab124ba8b0e06d90c,
title = "Salt stress alters DNA methylation levels in alfalfa (Medicago spp)",
abstract = "Modification of DNA methylation status is one of the mechanisms used by plants to adjust gene expression at both the transcriptional and posttranscriptional levels when plants are exposed to suboptimal conditions. Under abiotic stress, different cultivars often show heritable phenotypic variation accompanied by epigenetic polymorphisms at the DNA methylation level. This variation may provide the raw materials for plant breeding programs that aim to enhance abiotic stress tolerance, including salt tolerance. In this study, methylation-sensitive amplified polymorphism (MSAP) analysis was used to assess cytosine methylation levels in alfalfa (Medicago spp) roots exposed to increasing NaCl concentrations (0.0, 8.0, 12.0, and 20.0 dS/m). Eleven indigenous landraces were analyzed, in addition to a salt-tolerant cultivar that was used as a control. There was a slight increase in DNA methylation upon exposure to high levels of soil salinity. Phylogenetic analysis using MSAP showed epigenetic variation within and between the alfalfa landraces when exposed to saline conditions. Based on MSAP and enzyme-linked immunosorbent assay results, we found that salinity increased global DNA methylation status, particularly in plants exposed to the highest level of salinity (20 dS/m). Quantitative reverse transcription-polymerase chain reaction indicated that this might be mediated by the overexpression of methyltransferase homolog genes after exposure to saline conditions. DNA demethylation using 5-azacytidine reduced seedling lengths and dry and fresh weights, indicating a possible decrease in salinity tolerance. These results suggest that salinity affects DNA methylation flexibility.",
keywords = "Medicago, Methylation, MSAP, Salinity",
author = "A. Al-Lawati and S. Al-Bahry and R. Victor and Al-Lawati, {A. H.} and Yaish, {M. W.}",
year = "2016",
month = "2",
day = "26",
doi = "10.4238/gmr.15018299",
language = "English",
volume = "15",
journal = "Genetics and Molecular Research",
issn = "1676-5680",
publisher = "Fundacao de Pesquisas Cientificas de Ribeirao Preto",
number = "1",

}

TY - JOUR

T1 - Salt stress alters DNA methylation levels in alfalfa (Medicago spp)

AU - Al-Lawati, A.

AU - Al-Bahry, S.

AU - Victor, R.

AU - Al-Lawati, A. H.

AU - Yaish, M. W.

PY - 2016/2/26

Y1 - 2016/2/26

N2 - Modification of DNA methylation status is one of the mechanisms used by plants to adjust gene expression at both the transcriptional and posttranscriptional levels when plants are exposed to suboptimal conditions. Under abiotic stress, different cultivars often show heritable phenotypic variation accompanied by epigenetic polymorphisms at the DNA methylation level. This variation may provide the raw materials for plant breeding programs that aim to enhance abiotic stress tolerance, including salt tolerance. In this study, methylation-sensitive amplified polymorphism (MSAP) analysis was used to assess cytosine methylation levels in alfalfa (Medicago spp) roots exposed to increasing NaCl concentrations (0.0, 8.0, 12.0, and 20.0 dS/m). Eleven indigenous landraces were analyzed, in addition to a salt-tolerant cultivar that was used as a control. There was a slight increase in DNA methylation upon exposure to high levels of soil salinity. Phylogenetic analysis using MSAP showed epigenetic variation within and between the alfalfa landraces when exposed to saline conditions. Based on MSAP and enzyme-linked immunosorbent assay results, we found that salinity increased global DNA methylation status, particularly in plants exposed to the highest level of salinity (20 dS/m). Quantitative reverse transcription-polymerase chain reaction indicated that this might be mediated by the overexpression of methyltransferase homolog genes after exposure to saline conditions. DNA demethylation using 5-azacytidine reduced seedling lengths and dry and fresh weights, indicating a possible decrease in salinity tolerance. These results suggest that salinity affects DNA methylation flexibility.

AB - Modification of DNA methylation status is one of the mechanisms used by plants to adjust gene expression at both the transcriptional and posttranscriptional levels when plants are exposed to suboptimal conditions. Under abiotic stress, different cultivars often show heritable phenotypic variation accompanied by epigenetic polymorphisms at the DNA methylation level. This variation may provide the raw materials for plant breeding programs that aim to enhance abiotic stress tolerance, including salt tolerance. In this study, methylation-sensitive amplified polymorphism (MSAP) analysis was used to assess cytosine methylation levels in alfalfa (Medicago spp) roots exposed to increasing NaCl concentrations (0.0, 8.0, 12.0, and 20.0 dS/m). Eleven indigenous landraces were analyzed, in addition to a salt-tolerant cultivar that was used as a control. There was a slight increase in DNA methylation upon exposure to high levels of soil salinity. Phylogenetic analysis using MSAP showed epigenetic variation within and between the alfalfa landraces when exposed to saline conditions. Based on MSAP and enzyme-linked immunosorbent assay results, we found that salinity increased global DNA methylation status, particularly in plants exposed to the highest level of salinity (20 dS/m). Quantitative reverse transcription-polymerase chain reaction indicated that this might be mediated by the overexpression of methyltransferase homolog genes after exposure to saline conditions. DNA demethylation using 5-azacytidine reduced seedling lengths and dry and fresh weights, indicating a possible decrease in salinity tolerance. These results suggest that salinity affects DNA methylation flexibility.

KW - Medicago

KW - Methylation

KW - MSAP

KW - Salinity

UR - http://www.scopus.com/inward/record.url?scp=84961710258&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84961710258&partnerID=8YFLogxK

U2 - 10.4238/gmr.15018299

DO - 10.4238/gmr.15018299

M3 - Article

VL - 15

JO - Genetics and Molecular Research

JF - Genetics and Molecular Research

SN - 1676-5680

IS - 1

M1 - 15018299

ER -