Sacbrood virus of the honeybee (Apis mellifera)

Rapid identification and phylogenetic analysis using reverse transcription-PCR

E. Grabensteiner, W. Ritter, M. J. Carter, S. Davison, H. Pechhacker, J. Kolodziejek, O. Boecking, I. Derakhshifar, R. Moosbeckhofer, E. Licek, N. Nowotny

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Sacbrood virus (SBV) infects larvae of the honeybee (Apis mellifera), resulting in failure to pupate and death. Until now, identification of viruses in honeybee infections has been based on traditional methods such as electron microscopy, immunodiffusion, and enzyme-linked immunosorbent assay. Culture cannot be used because no honeybee cell lines are available. These techniques are low in sensitivity and specificity. However, the complete nucleotide sequence of SBV has recently been determined, and with these data, we now report a reverse transcription-PCR (RT-PCR) test for the direct, rapid, and sensitive detection of these viruses. RT-PCR was used to target five different areas of the SBV genome using infected honeybees and larvae originating from geographically distinct regions. The RT-PCR assay proved to be a rapid, specific, and sensitive diagnostic tool for the direct detection of SBV nucleic acid in samples of infected honeybees and brood regardless of geographic origin. The amplification products were sequenced, and phylogenetic analysis suggested the existence of at least three distinct genotypes of SBV.

Original languageEnglish
Pages (from-to)93-104
Number of pages12
JournalClinical and Diagnostic Laboratory Immunology
Volume8
Issue number1
DOIs
Publication statusPublished - 2001

Fingerprint

Bees
Transcription
Viruses
Reverse Transcription
Polymerase Chain Reaction
Larva
Assays
Immunosorbents
Immunodiffusion
Cell culture
Nucleic Acids
Electron microscopy
Amplification
Electron Microscopy
Nucleotides
Genes
Enzyme-Linked Immunosorbent Assay
Genotype
Cells
Genome

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Sacbrood virus of the honeybee (Apis mellifera) : Rapid identification and phylogenetic analysis using reverse transcription-PCR. / Grabensteiner, E.; Ritter, W.; Carter, M. J.; Davison, S.; Pechhacker, H.; Kolodziejek, J.; Boecking, O.; Derakhshifar, I.; Moosbeckhofer, R.; Licek, E.; Nowotny, N.

In: Clinical and Diagnostic Laboratory Immunology, Vol. 8, No. 1, 2001, p. 93-104.

Research output: Contribution to journalArticle

Grabensteiner, E, Ritter, W, Carter, MJ, Davison, S, Pechhacker, H, Kolodziejek, J, Boecking, O, Derakhshifar, I, Moosbeckhofer, R, Licek, E & Nowotny, N 2001, 'Sacbrood virus of the honeybee (Apis mellifera): Rapid identification and phylogenetic analysis using reverse transcription-PCR', Clinical and Diagnostic Laboratory Immunology, vol. 8, no. 1, pp. 93-104. https://doi.org/10.1128/CDLI.8.1.93-104.2001
Grabensteiner, E. ; Ritter, W. ; Carter, M. J. ; Davison, S. ; Pechhacker, H. ; Kolodziejek, J. ; Boecking, O. ; Derakhshifar, I. ; Moosbeckhofer, R. ; Licek, E. ; Nowotny, N. / Sacbrood virus of the honeybee (Apis mellifera) : Rapid identification and phylogenetic analysis using reverse transcription-PCR. In: Clinical and Diagnostic Laboratory Immunology. 2001 ; Vol. 8, No. 1. pp. 93-104.
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