RNA interference-mediated resistance to Tobacco streak virus in transgenic peanut

C. Senthilraja, M. Gurivi Reddy, J. Rajeswaran, E. Kokiladevi, R. Velazhahan

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Peanut stem necrosis disease (PSND) caused by Tobacco streak virus (TSV) is one of the important diseases of peanut (Arachis hypogaea L.) worldwide. In this study, we investigated the possibility of developing PSND- resistant transgenic peanut plants using RNAi technique. Peanut cultivar TMV-7 was transformed with a hairpin RNA (hpRNA) vector, pART27-TSV-IR-CP, containing inverted repeats of TSV-coat protein (CP) gene sequence flanking a spacer sequence (Pdk Intron) under the control of the CaMV 35S promoter using Agrobacterium tumefaciens -mediated transformation method. The presence of the TSV CP gene sequence in T0, T1 and T2 transgenic plants was confirmed by PCR analysis using CP gene specific primers. Evaluation of the transgenic plants (T2 generation) for resistance to TSV by using sap inoculation method revealed that none of the inoculated transgenic plants showed symptoms of infection, whereas non-transgenic control plants showed necrosis of leaves 7–9 days after inoculation and stem necrosis 16–19 days after inoculation. This study suggests that resistance against TSV can be induced in peanut plants through genetic engineering by expressing dsRNA of coat protein gene of TSV as target for RNAi.

Original languageEnglish
Pages (from-to)227-230
Number of pages4
JournalAustralasian Plant Pathology
Volume47
Issue number2
DOIs
Publication statusPublished - Mar 1 2018

Fingerprint

Tobacco streak virus
RNA interference
peanuts
coat proteins
genetically modified organisms
necrosis
transgenic plants
stems
nucleotide sequences
inoculation methods
plant genetics
double-stranded RNA
Arachis hypogaea
Agrobacterium radiobacter
genetic engineering
sap
signs and symptoms (plants)
introns
genes
promoter regions

Keywords

  • Arachis hypogaea
  • coat protein gene
  • RNA interference
  • Tobacco streak virus

ASJC Scopus subject areas

  • Plant Science

Cite this

RNA interference-mediated resistance to Tobacco streak virus in transgenic peanut. / Senthilraja, C.; Gurivi Reddy, M.; Rajeswaran, J.; Kokiladevi, E.; Velazhahan, R.

In: Australasian Plant Pathology, Vol. 47, No. 2, 01.03.2018, p. 227-230.

Research output: Contribution to journalArticle

Senthilraja, C. ; Gurivi Reddy, M. ; Rajeswaran, J. ; Kokiladevi, E. ; Velazhahan, R. / RNA interference-mediated resistance to Tobacco streak virus in transgenic peanut. In: Australasian Plant Pathology. 2018 ; Vol. 47, No. 2. pp. 227-230.
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