RNA in situ hybridization for the detection of feline immunodeficiency virus in infected cells

G. Ryan; C. O'Flatharta; J. Callanan; Mohamed J.E.M.F. Mabruk

RNA in situ hybridization for the detection of feline immunodeficiency virus in infected cells

G. Ryan, C. O'Flatharta, J. Callanan, Mohamed J.E.M.F. Mabruk^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

In the present study, we have applied a novel approach to generate specific digoxigenin- and biotin-labeled RNA probes to detect Feline Immunodeficiency Virus (FIV) gag gene in the FIV-infected feline T-lymphoblastoid cell line (MYA-1). This involved cloning of the FIV gag gene into a PCR Script vector containing both T3 and T7 promoters, followed by amplification of the insert and the two promoter sequences, using specific primer sequences. The FIV RNA probes were more sensitive than FIV DNA probes. This approach should make RNA in situ hybridization more accessible for use in routine diagnosis.

Original language	English
Pages (from-to)	106-112
Number of pages	7
Journal	Southeast Asian Journal of Tropical Medicine and Public Health
Volume	37
Issue number	1
Publication status	Published - Jan 2006
Externally published	Yes

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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AU - Callanan, J.

AU - Mabruk, Mohamed J.E.M.F.

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AB - In the present study, we have applied a novel approach to generate specific digoxigenin- and biotin-labeled RNA probes to detect Feline Immunodeficiency Virus (FIV) gag gene in the FIV-infected feline T-lymphoblastoid cell line (MYA-1). This involved cloning of the FIV gag gene into a PCR Script vector containing both T3 and T7 promoters, followed by amplification of the insert and the two promoter sequences, using specific primer sequences. The FIV RNA probes were more sensitive than FIV DNA probes. This approach should make RNA in situ hybridization more accessible for use in routine diagnosis.

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RNA in situ hybridization for the detection of feline immunodeficiency virus in infected cells

Abstract

ASJC Scopus subject areas

UN SDGs

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