RNA in situ hybridization for the detection of feline immunodeficiency virus in infected cells

G. Ryan, C. O'Flatharta, J. Callanan, Mohamed J E M F Mabruk

Research output: Contribution to journalArticle

Abstract

In the present study, we have applied a novel approach to generate specific digoxigenin- and biotin-labeled RNA probes to detect Feline Immunodeficiency Virus (FIV) gag gene in the FIV-infected feline T-lymphoblastoid cell line (MYA-1). This involved cloning of the FIV gag gene into a PCR Script vector containing both T3 and T7 promoters, followed by amplification of the insert and the two promoter sequences, using specific primer sequences. The FIV RNA probes were more sensitive than FIV DNA probes. This approach should make RNA in situ hybridization more accessible for use in routine diagnosis.

Original languageEnglish
Pages (from-to)106-112
Number of pages7
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume37
Issue number1
Publication statusPublished - Jan 2006

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Feline Immunodeficiency Virus
In Situ Hybridization
RNA
gag Genes
RNA Probes
Digoxigenin
Felidae
DNA Probes
Biotin
Organism Cloning
Cell Line
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Medicine(all)

Cite this

RNA in situ hybridization for the detection of feline immunodeficiency virus in infected cells. / Ryan, G.; O'Flatharta, C.; Callanan, J.; Mabruk, Mohamed J E M F.

In: Southeast Asian Journal of Tropical Medicine and Public Health, Vol. 37, No. 1, 01.2006, p. 106-112.

Research output: Contribution to journalArticle

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