TY - JOUR
T1 - Reversed phase High Performance Liquid Chromatography used for the physicochemical and thermodynamic characterization of piceatannol/β-cyclodextrin complex
AU - Messiad, Hanane
AU - Amira-Guebailia, Habiba
AU - Houache, Omar
PY - 2013/5/1
Y1 - 2013/5/1
N2 - Piceatannol is one of resveratrol derivatives having health promoting potential. However, its low water-solubility and bioavailability could limit its use in both food and pharmaceutical fields. The aim of this work is the study of piceatannol complexation by β-cyclodextrin (β-CD) in aqueous media. The complex formed could improve the bioavailability, the solubility and the stability of piceatannol. The method used was based on RP-HPLC in which, β-CD was added to methanol/water mixtures mobile phases. The apparent formation constant of piceatannol/β-cyclodextrin complex was determined. Within the concentration interval studied (0-4mM), whenever the concentration of β-CD increased, retention time of piceatannol decreased; indicating an enhancement of solubility due to the formation of the piceatannol/β-CD complex. The formation constants (KF) of the piceatannol/β-CD complex varied significantly with both the methanol concentration in the water-methanol mixture and the temperature. Moreover, in all the physicochemical conditions tested, the stoichiometry of piceatannol/β-CD complex was 1:1. Finally, the thermodynamic parameters were determined: ΔG°=-13.123kJmol-1, ΔH°=-33. 265kJmol-1 and ΔS°=-67.559Jmol-1K-1.
AB - Piceatannol is one of resveratrol derivatives having health promoting potential. However, its low water-solubility and bioavailability could limit its use in both food and pharmaceutical fields. The aim of this work is the study of piceatannol complexation by β-cyclodextrin (β-CD) in aqueous media. The complex formed could improve the bioavailability, the solubility and the stability of piceatannol. The method used was based on RP-HPLC in which, β-CD was added to methanol/water mixtures mobile phases. The apparent formation constant of piceatannol/β-cyclodextrin complex was determined. Within the concentration interval studied (0-4mM), whenever the concentration of β-CD increased, retention time of piceatannol decreased; indicating an enhancement of solubility due to the formation of the piceatannol/β-CD complex. The formation constants (KF) of the piceatannol/β-CD complex varied significantly with both the methanol concentration in the water-methanol mixture and the temperature. Moreover, in all the physicochemical conditions tested, the stoichiometry of piceatannol/β-CD complex was 1:1. Finally, the thermodynamic parameters were determined: ΔG°=-13.123kJmol-1, ΔH°=-33. 265kJmol-1 and ΔS°=-67.559Jmol-1K-1.
KW - Apparent formation constant
KW - Mobile phase
KW - Piceatannol
KW - Solubility
KW - Thermodynamic parameters
KW - β-Cyclodextrin
UR - http://www.scopus.com/inward/record.url?scp=84875654878&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875654878&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2013.02.024
DO - 10.1016/j.jchromb.2013.02.024
M3 - Article
C2 - 23537743
AN - SCOPUS:84875654878
SN - 1570-0232
VL - 926
SP - 21
EP - 27
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ER -