Responses of alveolar macrophages to post-Gulf-War airborne dust from Kuwait

Charles I. Ezeamuzie, Mirza U. Beg, Dhari Al-Ajmi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Kuwait oil fires of 1991 resulting from the Iraqi invasion created an unprecedented environmental pollution to which the population was exposed for a prolonged period. The impact of such pollution on human health, especially the respiratory system, needs to be adequately assessed, given the well known relationship between air pollution and respiratory diseases. Alveolar macrophages (AM) are the main resident cells in the bronchial lumen and are known to perform microbicidal and tumoricidal roles by releasing oxygen radicals and cytokines. The effects of the extracts and particles of the post oil fire airborne dust collected from air conditioner filters in the Ahmadi area of Kuwait in 1991-1995 on the in vitro spontaneous and PMA- stimulated generation of superoxide ions (O2·) from guinea pig AM and lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNFα) release from mouse AM were studied. Treatment of adherent AM with aqueous extract of the 1991 dust (0.6-10 mg/mL) resulted in a dose-dependent inhibition of basal, as well as PMA-induced O 2· release. At lower concentrations, slight increases were seen. Fifty percent inhibition of O 2· release was achieved by extracts of 1-2.4 mg/mL of the dust and at 10 mg/mL the maximal inhibition was 79-88%. At the same concentration range, the extract also inhibited the LPS-stimulated release of TNFα from mouse AM. These effects were more pronounced in the 1991 dust samples than in the 1992 and 1995 samples. No such effect was seen with the extract of silica (SiO 2) used as particulate control. All washed dust or SiO 2 particulates directly induced the release of both O 2· and TNFα from these cells. These results show that the post- oil fire airborne dust of Kuwait contained soluble pollutants that were capable of inhibiting the host defensive functions of the AM and this may have possibly resulted in an increased susceptibility to respiratory infections and neoplasms among the exposed population.

Original languageEnglish
Pages (from-to)213-220
Number of pages8
JournalEnvironment International
Volume24
Issue number1-2
DOIs
Publication statusPublished - Jan 1998

Fingerprint

Dust
dust
tumor
Oils
Fires
Tumor Necrosis Factor-alpha
Lipopolysaccharides
oil
Pollution
Respiratory system
Pulmonary diseases
respiratory disease
Air pollution
Superoxides
pig
Silicon Dioxide
Particles (particulate matter)
Alveolar Macrophages
gulf
Reactive Oxygen Species

ASJC Scopus subject areas

  • Environmental Chemistry
  • Environmental Science(all)

Cite this

Responses of alveolar macrophages to post-Gulf-War airborne dust from Kuwait. / Ezeamuzie, Charles I.; Beg, Mirza U.; Al-Ajmi, Dhari.

In: Environment International, Vol. 24, No. 1-2, 01.1998, p. 213-220.

Research output: Contribution to journalArticle

Ezeamuzie, Charles I. ; Beg, Mirza U. ; Al-Ajmi, Dhari. / Responses of alveolar macrophages to post-Gulf-War airborne dust from Kuwait. In: Environment International. 1998 ; Vol. 24, No. 1-2. pp. 213-220.
@article{a48dfa1226b54a65ba0f6c245606eb1f,
title = "Responses of alveolar macrophages to post-Gulf-War airborne dust from Kuwait",
abstract = "The Kuwait oil fires of 1991 resulting from the Iraqi invasion created an unprecedented environmental pollution to which the population was exposed for a prolonged period. The impact of such pollution on human health, especially the respiratory system, needs to be adequately assessed, given the well known relationship between air pollution and respiratory diseases. Alveolar macrophages (AM) are the main resident cells in the bronchial lumen and are known to perform microbicidal and tumoricidal roles by releasing oxygen radicals and cytokines. The effects of the extracts and particles of the post oil fire airborne dust collected from air conditioner filters in the Ahmadi area of Kuwait in 1991-1995 on the in vitro spontaneous and PMA- stimulated generation of superoxide ions (O2·) from guinea pig AM and lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNFα) release from mouse AM were studied. Treatment of adherent AM with aqueous extract of the 1991 dust (0.6-10 mg/mL) resulted in a dose-dependent inhibition of basal, as well as PMA-induced O 2· release. At lower concentrations, slight increases were seen. Fifty percent inhibition of O 2· release was achieved by extracts of 1-2.4 mg/mL of the dust and at 10 mg/mL the maximal inhibition was 79-88{\%}. At the same concentration range, the extract also inhibited the LPS-stimulated release of TNFα from mouse AM. These effects were more pronounced in the 1991 dust samples than in the 1992 and 1995 samples. No such effect was seen with the extract of silica (SiO 2) used as particulate control. All washed dust or SiO 2 particulates directly induced the release of both O 2· and TNFα from these cells. These results show that the post- oil fire airborne dust of Kuwait contained soluble pollutants that were capable of inhibiting the host defensive functions of the AM and this may have possibly resulted in an increased susceptibility to respiratory infections and neoplasms among the exposed population.",
author = "Ezeamuzie, {Charles I.} and Beg, {Mirza U.} and Dhari Al-Ajmi",
year = "1998",
month = "1",
doi = "10.1016/S0160-4120(97)00138-4",
language = "English",
volume = "24",
pages = "213--220",
journal = "Environmental International",
issn = "0160-4120",
publisher = "Elsevier Limited",
number = "1-2",

}

TY - JOUR

T1 - Responses of alveolar macrophages to post-Gulf-War airborne dust from Kuwait

AU - Ezeamuzie, Charles I.

AU - Beg, Mirza U.

AU - Al-Ajmi, Dhari

PY - 1998/1

Y1 - 1998/1

N2 - The Kuwait oil fires of 1991 resulting from the Iraqi invasion created an unprecedented environmental pollution to which the population was exposed for a prolonged period. The impact of such pollution on human health, especially the respiratory system, needs to be adequately assessed, given the well known relationship between air pollution and respiratory diseases. Alveolar macrophages (AM) are the main resident cells in the bronchial lumen and are known to perform microbicidal and tumoricidal roles by releasing oxygen radicals and cytokines. The effects of the extracts and particles of the post oil fire airborne dust collected from air conditioner filters in the Ahmadi area of Kuwait in 1991-1995 on the in vitro spontaneous and PMA- stimulated generation of superoxide ions (O2·) from guinea pig AM and lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNFα) release from mouse AM were studied. Treatment of adherent AM with aqueous extract of the 1991 dust (0.6-10 mg/mL) resulted in a dose-dependent inhibition of basal, as well as PMA-induced O 2· release. At lower concentrations, slight increases were seen. Fifty percent inhibition of O 2· release was achieved by extracts of 1-2.4 mg/mL of the dust and at 10 mg/mL the maximal inhibition was 79-88%. At the same concentration range, the extract also inhibited the LPS-stimulated release of TNFα from mouse AM. These effects were more pronounced in the 1991 dust samples than in the 1992 and 1995 samples. No such effect was seen with the extract of silica (SiO 2) used as particulate control. All washed dust or SiO 2 particulates directly induced the release of both O 2· and TNFα from these cells. These results show that the post- oil fire airborne dust of Kuwait contained soluble pollutants that were capable of inhibiting the host defensive functions of the AM and this may have possibly resulted in an increased susceptibility to respiratory infections and neoplasms among the exposed population.

AB - The Kuwait oil fires of 1991 resulting from the Iraqi invasion created an unprecedented environmental pollution to which the population was exposed for a prolonged period. The impact of such pollution on human health, especially the respiratory system, needs to be adequately assessed, given the well known relationship between air pollution and respiratory diseases. Alveolar macrophages (AM) are the main resident cells in the bronchial lumen and are known to perform microbicidal and tumoricidal roles by releasing oxygen radicals and cytokines. The effects of the extracts and particles of the post oil fire airborne dust collected from air conditioner filters in the Ahmadi area of Kuwait in 1991-1995 on the in vitro spontaneous and PMA- stimulated generation of superoxide ions (O2·) from guinea pig AM and lipopolysaccharide (LPS)-stimulated tumor necrosis factor (TNFα) release from mouse AM were studied. Treatment of adherent AM with aqueous extract of the 1991 dust (0.6-10 mg/mL) resulted in a dose-dependent inhibition of basal, as well as PMA-induced O 2· release. At lower concentrations, slight increases were seen. Fifty percent inhibition of O 2· release was achieved by extracts of 1-2.4 mg/mL of the dust and at 10 mg/mL the maximal inhibition was 79-88%. At the same concentration range, the extract also inhibited the LPS-stimulated release of TNFα from mouse AM. These effects were more pronounced in the 1991 dust samples than in the 1992 and 1995 samples. No such effect was seen with the extract of silica (SiO 2) used as particulate control. All washed dust or SiO 2 particulates directly induced the release of both O 2· and TNFα from these cells. These results show that the post- oil fire airborne dust of Kuwait contained soluble pollutants that were capable of inhibiting the host defensive functions of the AM and this may have possibly resulted in an increased susceptibility to respiratory infections and neoplasms among the exposed population.

UR - http://www.scopus.com/inward/record.url?scp=0031933916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031933916&partnerID=8YFLogxK

U2 - 10.1016/S0160-4120(97)00138-4

DO - 10.1016/S0160-4120(97)00138-4

M3 - Article

VL - 24

SP - 213

EP - 220

JO - Environmental International

JF - Environmental International

SN - 0160-4120

IS - 1-2

ER -