Reference ranges for lymphocyte subsets in healthy adult male Omanis.

Ali A. Al-Jabri, Ahmed K. Al-Shukaili, Zowaina T. Al-Rashdi, Shyam S. Ganguly

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. METHODS: A cohort, of 118 healthy male blood donors ranging in age from 18-51 years, was included in the study. The average age was 25 years. Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets using flow cytometer. This study was conducted in the Immunology Laboratory of the Sultan Qaboos University, College of Medicine and Health Sciences, Muscat, Oman during the year 2006. RESULTS: For the 118 males investigated, the mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 68.53 +/- 7.5%, 1701 +/- 489 cells/microliter; CD4: 40.4 +/- 6.5%, 1006 +/- 319 cells/microliter; CD8: 25.8 +/- 5.9%, 638 +/- 225 cells/microliter; CD19: 13.7 +/- 4.7%, 349 +/- 158 cells/microliter, and CD56: 12.2 +/- 6.7%, 308 +/- 204 cells/microliter. The ratio of CD4/CD8 was 1.6. CONCLUSION: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adult males in Oman. The Omani male reference values obtained in this study show wide variations compared with kits values previously used as a reference.

Original languageEnglish
Pages (from-to)409-412
Number of pages4
JournalSaudi Medical Journal
Volume29
Issue number3
Publication statusPublished - Mar 2008

Fingerprint

Lymphocyte Subsets
Oman
Reference Values
Immunophenotyping
CD4-CD8 Ratio
Diamines
Allergy and Immunology
Blood Donors
Acetic Acid
Medicine
HIV
Health

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Reference ranges for lymphocyte subsets in healthy adult male Omanis. / Al-Jabri, Ali A.; Al-Shukaili, Ahmed K.; Al-Rashdi, Zowaina T.; Ganguly, Shyam S.

In: Saudi Medical Journal, Vol. 29, No. 3, 03.2008, p. 409-412.

Research output: Contribution to journalArticle

Al-Jabri, Ali A. ; Al-Shukaili, Ahmed K. ; Al-Rashdi, Zowaina T. ; Ganguly, Shyam S. / Reference ranges for lymphocyte subsets in healthy adult male Omanis. In: Saudi Medical Journal. 2008 ; Vol. 29, No. 3. pp. 409-412.
@article{25146da6bc3f4d05995de106ae1534c0,
title = "Reference ranges for lymphocyte subsets in healthy adult male Omanis.",
abstract = "OBJECTIVE: To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. METHODS: A cohort, of 118 healthy male blood donors ranging in age from 18-51 years, was included in the study. The average age was 25 years. Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets using flow cytometer. This study was conducted in the Immunology Laboratory of the Sultan Qaboos University, College of Medicine and Health Sciences, Muscat, Oman during the year 2006. RESULTS: For the 118 males investigated, the mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 68.53 +/- 7.5{\%}, 1701 +/- 489 cells/microliter; CD4: 40.4 +/- 6.5{\%}, 1006 +/- 319 cells/microliter; CD8: 25.8 +/- 5.9{\%}, 638 +/- 225 cells/microliter; CD19: 13.7 +/- 4.7{\%}, 349 +/- 158 cells/microliter, and CD56: 12.2 +/- 6.7{\%}, 308 +/- 204 cells/microliter. The ratio of CD4/CD8 was 1.6. CONCLUSION: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adult males in Oman. The Omani male reference values obtained in this study show wide variations compared with kits values previously used as a reference.",
author = "Al-Jabri, {Ali A.} and Al-Shukaili, {Ahmed K.} and Al-Rashdi, {Zowaina T.} and Ganguly, {Shyam S.}",
year = "2008",
month = "3",
language = "English",
volume = "29",
pages = "409--412",
journal = "Saudi Medical Journal",
issn = "0379-5284",
publisher = "Saudi Arabian Armed Forces Hospital",
number = "3",

}

TY - JOUR

T1 - Reference ranges for lymphocyte subsets in healthy adult male Omanis.

AU - Al-Jabri, Ali A.

AU - Al-Shukaili, Ahmed K.

AU - Al-Rashdi, Zowaina T.

AU - Ganguly, Shyam S.

PY - 2008/3

Y1 - 2008/3

N2 - OBJECTIVE: To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. METHODS: A cohort, of 118 healthy male blood donors ranging in age from 18-51 years, was included in the study. The average age was 25 years. Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets using flow cytometer. This study was conducted in the Immunology Laboratory of the Sultan Qaboos University, College of Medicine and Health Sciences, Muscat, Oman during the year 2006. RESULTS: For the 118 males investigated, the mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 68.53 +/- 7.5%, 1701 +/- 489 cells/microliter; CD4: 40.4 +/- 6.5%, 1006 +/- 319 cells/microliter; CD8: 25.8 +/- 5.9%, 638 +/- 225 cells/microliter; CD19: 13.7 +/- 4.7%, 349 +/- 158 cells/microliter, and CD56: 12.2 +/- 6.7%, 308 +/- 204 cells/microliter. The ratio of CD4/CD8 was 1.6. CONCLUSION: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adult males in Oman. The Omani male reference values obtained in this study show wide variations compared with kits values previously used as a reference.

AB - OBJECTIVE: To determine the reference ranges of lymphocyte subsets in serologically HIV-seronegative healthy male adults in Oman. METHODS: A cohort, of 118 healthy male blood donors ranging in age from 18-51 years, was included in the study. The average age was 25 years. Blood samples collected into tubes containing ethylene-diamine-tetra acetic acid were investigated for lymphocyte subsets using flow cytometer. This study was conducted in the Immunology Laboratory of the Sultan Qaboos University, College of Medicine and Health Sciences, Muscat, Oman during the year 2006. RESULTS: For the 118 males investigated, the mean percentage and absolute values of the lymphocyte subsets were as follows: CD3: 68.53 +/- 7.5%, 1701 +/- 489 cells/microliter; CD4: 40.4 +/- 6.5%, 1006 +/- 319 cells/microliter; CD8: 25.8 +/- 5.9%, 638 +/- 225 cells/microliter; CD19: 13.7 +/- 4.7%, 349 +/- 158 cells/microliter, and CD56: 12.2 +/- 6.7%, 308 +/- 204 cells/microliter. The ratio of CD4/CD8 was 1.6. CONCLUSION: Immunophenotyping has been used to establish reference values of lymphocyte subsets in normal healthy adult males in Oman. The Omani male reference values obtained in this study show wide variations compared with kits values previously used as a reference.

UR - http://www.scopus.com/inward/record.url?scp=47549091384&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47549091384&partnerID=8YFLogxK

M3 - Article

C2 - 18327369

AN - SCOPUS:47549091384

VL - 29

SP - 409

EP - 412

JO - Saudi Medical Journal

JF - Saudi Medical Journal

SN - 0379-5284

IS - 3

ER -