Reactivity of Schistosoma japonicum and S. Mansoni antigen preparations in indirect haemagglutination (IHA) with sera of patients with homologous and heterologous schistosomiasis

M. Gui, M. A. Idris, Y. E. Shi, A. Muhling, A. Ruppel

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Sera of patients infected with Schistosoma japonicum, S. mansoni or S. haematobium were tested in an indirect haemagglutination assay (IHA) using soluble S. japonicum egg antigen (SjSEA) and soluble S. mansoni adult antigen prepared either from a Puerto Rican strain (SmAWA) or an Egyptian strain (SmBW; Cellognost(®)-Schistosomiasis Kit). Reactions were best, in terms of titres and sensitivity, in homologous systems. Heterologous systems were less reliable, particularly those using sera from urinary schistosomiasis patients. It is suggested that IHA is a suitable test to detect Schistosoma infections, especially when homologous systems are used.

Original languageEnglish
Pages (from-to)599-604
Number of pages6
JournalAnnals of Tropical Medicine and Parasitology
Volume85
Issue number6
Publication statusPublished - 1991

Fingerprint

Schistosoma japonicum
Schistosomiasis
Hemagglutination
Schistosomiasis haematobia
Schistosoma
Antigens
Serum
Ovum
Infection

ASJC Scopus subject areas

  • Immunology
  • Parasitology
  • Infectious Diseases

Cite this

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abstract = "Sera of patients infected with Schistosoma japonicum, S. mansoni or S. haematobium were tested in an indirect haemagglutination assay (IHA) using soluble S. japonicum egg antigen (SjSEA) and soluble S. mansoni adult antigen prepared either from a Puerto Rican strain (SmAWA) or an Egyptian strain (SmBW; Cellognost({\circledR})-Schistosomiasis Kit). Reactions were best, in terms of titres and sensitivity, in homologous systems. Heterologous systems were less reliable, particularly those using sera from urinary schistosomiasis patients. It is suggested that IHA is a suitable test to detect Schistosoma infections, especially when homologous systems are used.",
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T1 - Reactivity of Schistosoma japonicum and S. Mansoni antigen preparations in indirect haemagglutination (IHA) with sera of patients with homologous and heterologous schistosomiasis

AU - Gui, M.

AU - Idris, M. A.

AU - Shi, Y. E.

AU - Muhling, A.

AU - Ruppel, A.

PY - 1991

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AB - Sera of patients infected with Schistosoma japonicum, S. mansoni or S. haematobium were tested in an indirect haemagglutination assay (IHA) using soluble S. japonicum egg antigen (SjSEA) and soluble S. mansoni adult antigen prepared either from a Puerto Rican strain (SmAWA) or an Egyptian strain (SmBW; Cellognost(®)-Schistosomiasis Kit). Reactions were best, in terms of titres and sensitivity, in homologous systems. Heterologous systems were less reliable, particularly those using sera from urinary schistosomiasis patients. It is suggested that IHA is a suitable test to detect Schistosoma infections, especially when homologous systems are used.

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