Rapid detection of Ganoderma lucidum and assessment of inhibition effect of various control measures by immunoassay and PCR

M. Karthikeyan, K. Radhika, R. Bhaskaran, S. Mathiyazhagan, R. Velazhahan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Molecular and immunological methods have been applied for detecting the Ganoderma disease of coconut. Polyclonal antibodies (PAbs) raised against basidiocarp protein of Ganoderma were used for detection. For polymerase chain reaction (PCR) test, the primer generated from the internal transcribed spacer region one (ITS 1) of ribosomal DNA gene of Ganoderma, which produced a PCR product of 167 bp in size is used for early detection. Ganoderma disease in apparently healthy palms in two coconut gardens was tested by ELISA test using basidiocarp protein antiserum. Field trials were laid out in these early-diagnosed palms for the management of the disease. Based on the ELISA results, Pseudomonas fluorescens + Trichoderma viride with chitin amended treatments arrested the multiplication of the pathogen and showed below the infection level of optical density (O.D) within six months. Integrated disease management (IDM) and fungicide tridemorph treated palms showed below infection level (O.D value) within seven months and T. harzianum and P. fluorescens + T. viride treated palms showed below infection level (OD value) of the disease in eighth months.

Original languageEnglish
Pages (from-to)2202-2208
Number of pages7
JournalAfrican Journal of Biotechnology
Volume8
Issue number10
Publication statusPublished - May 18 2009

Fingerprint

Ganoderma
Reishi
Ganoderma lucidum
immunoassays
Immunoassay
control methods
Fungal Fruiting Bodies
polymerase chain reaction
Pseudomonas fluorescens
Polymerase Chain Reaction
basidiomata
Cocos
Trichoderma viride
coconuts
Disease Management
absorbance
disease control
tridemorph
Infection
Enzyme-Linked Immunosorbent Assay

Keywords

  • Early diagnose
  • ELISA
  • Ganoderma
  • Integrated disease management
  • PCR

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics

Cite this

Rapid detection of Ganoderma lucidum and assessment of inhibition effect of various control measures by immunoassay and PCR. / Karthikeyan, M.; Radhika, K.; Bhaskaran, R.; Mathiyazhagan, S.; Velazhahan, R.

In: African Journal of Biotechnology, Vol. 8, No. 10, 18.05.2009, p. 2202-2208.

Research output: Contribution to journalArticle

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