Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays

Jeremy V. Camp; Norbert Nowotny

doi:10.1016/j.jviromet.2016.08.001

Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays

Jeremy V. Camp^*, Norbert Nowotny

^*Corresponding author for this work

Microbiology & Immunology

Research output: Contribution to journal › Article › peer-review

1 Citation (Scopus)

Abstract

The development of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assays are described herein for the detection of two orthobunyaviruses (Bunyaviridae), which represent the two main serogroups found in mosquitoes in Central Europe. The RT-LAMP assays were optimized for the detection of Ťahyňa virus (a California encephalitis group virus found in Aedes sp or Ochlerotatus sp mosquitoes) and Batai virus (also called Čalovo virus, a Bunyamwera group virus found in Anopheles maculipennis s.l. mosquitoes) nucleic acid using endemic European virus isolates. The sensitivity of the RT-LAMP assays was determined to be comparable to that of conventional tests, with a limit of detection

Original language	English
Pages (from-to)	252-257
Number of pages	6
Journal	Journal of Virological Methods
Volume	236
DOIs	https://doi.org/10.1016/j.jviromet.2016.08.001
Publication status	Published - Oct 1 2016

Keywords

Arbovirus
Bunyaviridae
Loop-mediated isothermal amplification
Mosquito
Orthobunyavirus
RT-LAMP

ASJC Scopus subject areas

Virology

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10.1016/j.jviromet.2016.08.001

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title = "Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays",

abstract = "The development of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assays are described herein for the detection of two orthobunyaviruses (Bunyaviridae), which represent the two main serogroups found in mosquitoes in Central Europe. The RT-LAMP assays were optimized for the detection of {\v T}ahy{\v n}a virus (a California encephalitis group virus found in Aedes sp or Ochlerotatus sp mosquitoes) and Batai virus (also called {\v C}alovo virus, a Bunyamwera group virus found in Anopheles maculipennis s.l. mosquitoes) nucleic acid using endemic European virus isolates. The sensitivity of the RT-LAMP assays was determined to be comparable to that of conventional tests, with a limit of detection ",

keywords = "Arbovirus, Bunyaviridae, Loop-mediated isothermal amplification, Mosquito, Orthobunyavirus, RT-LAMP",

author = "Camp, {Jeremy V.} and Norbert Nowotny",

year = "2016",

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doi = "10.1016/j.jviromet.2016.08.001",

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journal = "Journal of Virological Methods",

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publisher = "Elsevier",

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TY - JOUR

T1 - Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays

AU - Camp, Jeremy V.

AU - Nowotny, Norbert

PY - 2016/10/1

Y1 - 2016/10/1

N2 - The development of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assays are described herein for the detection of two orthobunyaviruses (Bunyaviridae), which represent the two main serogroups found in mosquitoes in Central Europe. The RT-LAMP assays were optimized for the detection of Ťahyňa virus (a California encephalitis group virus found in Aedes sp or Ochlerotatus sp mosquitoes) and Batai virus (also called Čalovo virus, a Bunyamwera group virus found in Anopheles maculipennis s.l. mosquitoes) nucleic acid using endemic European virus isolates. The sensitivity of the RT-LAMP assays was determined to be comparable to that of conventional tests, with a limit of detection

AB - The development of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assays are described herein for the detection of two orthobunyaviruses (Bunyaviridae), which represent the two main serogroups found in mosquitoes in Central Europe. The RT-LAMP assays were optimized for the detection of Ťahyňa virus (a California encephalitis group virus found in Aedes sp or Ochlerotatus sp mosquitoes) and Batai virus (also called Čalovo virus, a Bunyamwera group virus found in Anopheles maculipennis s.l. mosquitoes) nucleic acid using endemic European virus isolates. The sensitivity of the RT-LAMP assays was determined to be comparable to that of conventional tests, with a limit of detection

KW - Arbovirus

KW - Bunyaviridae

KW - Loop-mediated isothermal amplification

KW - Mosquito

KW - Orthobunyavirus

KW - RT-LAMP

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DO - 10.1016/j.jviromet.2016.08.001

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AN - SCOPUS:84981297739

SN - 0166-0934

VL - 236

SP - 252

EP - 257

JO - Journal of Virological Methods

JF - Journal of Virological Methods

ER -

Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays

Abstract

Keywords

ASJC Scopus subject areas

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