Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays

Jeremy V. Camp, Norbert Nowotny

Research output: Contribution to journalArticle

Abstract

The development of reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assays are described herein for the detection of two orthobunyaviruses (Bunyaviridae), which represent the two main serogroups found in mosquitoes in Central Europe. The RT-LAMP assays were optimized for the detection of Ťahyňa virus (a California encephalitis group virus found in Aedes sp or Ochlerotatus sp mosquitoes) and Batai virus (also called Čalovo virus, a Bunyamwera group virus found in Anopheles maculipennis s.l. mosquitoes) nucleic acid using endemic European virus isolates. The sensitivity of the RT-LAMP assays was determined to be comparable to that of conventional tests, with a limit of detection 

Original languageEnglish
Pages (from-to)252-257
Number of pages6
JournalJournal of Virological Methods
Volume236
DOIs
Publication statusPublished - Oct 1 2016

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Orthobunyavirus
RNA-Directed DNA Polymerase
Bunyamwera virus
California Encephalitis Viruses
Culicidae
Reverse Transcription
Viruses
Ochlerotatus
Bunyaviridae
Anopheles
Aedes
Nucleic Acids
Limit of Detection

Keywords

  • Arbovirus
  • Bunyaviridae
  • Loop-mediated isothermal amplification
  • Mosquito
  • Orthobunyavirus
  • RT-LAMP

ASJC Scopus subject areas

  • Virology

Cite this

Rapid detection of European orthobunyaviruses by reverse transcription loop-mediated isothermal amplification assays. / Camp, Jeremy V.; Nowotny, Norbert.

In: Journal of Virological Methods, Vol. 236, 01.10.2016, p. 252-257.

Research output: Contribution to journalArticle

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