Quorum-sensing inhibitory compounds from extremophilic microorganisms isolated from a hypersaline cyanobacterial mat

Raeid M M Abed, Sergey Dobretsov, Marwan Al-Fori, Sarath P. Gunasekera, Kumar Sudesh, Valerie J. Paul

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

In this study, extremely halophilic and moderately thermophilic microorganisms from a hypersaline microbial mat were screened for their ability to produce antibacterial, antidiatom, antialgal, and quorum-sensing (QS) inhibitory compounds. Five bacterial strains belonging to the genera Marinobacter and Halomonas and one archaeal strain belonging to the genus Haloterrigena were isolated from a microbial mat. The strains were able to grow at a maximum salinity of 22-25 % and a maximum temperature of 45-60 C. Hexanes, dichloromethane, and butanol extracts from the strains inhibited the growth of at least one out of nine human pathogens. Only butanol extracts of supernatants of Halomonas sp. SK-1 inhibited growth of the microalga Dunaliella salina. Most extracts from isolates inhibited QS of the acyl homoserine lactone producer and reporter Chromobacterium violaceum CV017. Purification of QS inhibitory dichloromethane extracts of Marinobacter sp. SK-3 resulted in isolation of four related diketopiperazines (DKPs): cyclo(l-Pro-l-Phe), cyclo(l-Pro-l-Leu), cyclo(l-Pro-l-isoLeu), and cyclo(l-Pro-d-Phe). QS inhibitory properties of these DKPs were tested using C. violaceum CV017 and Escherichia coli-based QS reporters (pSB401 and pSB1075) deficient in AHL production. Cyclo(l-Pro-l-Phe) and cyclo(l-Pro-l-isoLeu) inhibited QS-dependent production of violacein by C. violaceum CV017. Cyclo(l-Pro-l-Phe), cyclo(l-Pro-l-Leu), and cyclo(l-Pro-l-isoLeu) reduced QS-dependent luminescence of the reporter E. coli pSB401 induced by 3-oxo-C6-HSL. Our study demonstrated the ability of halophilic and moderately thermophilic strains from a hypersaline microbial mat to produce biotechnologically relevant compounds that could be used as antifouling agents.

Original languageEnglish
Pages (from-to)759-772
Number of pages14
JournalJournal of Industrial Microbiology and Biotechnology
Volume40
Issue number7
DOIs
Publication statusPublished - Jul 2013

Fingerprint

Quorum Sensing
Microorganisms
Diketopiperazines
Butanols
Marinobacter
Methylene Chloride
Halomonas
Dichloromethane
Butenes
Escherichia coli
Acyl-Butyrolactones
Hexanes
Chromobacterium
Pathogens
Hexane
Purification
Luminescence
Salinity
Growth
Temperature

Keywords

  • Antimicrobial, extreme halophiles
  • Biofouling
  • Cyanobacterial mats
  • Diketopiperazines
  • Quorum-sensing inhibition

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Quorum-sensing inhibitory compounds from extremophilic microorganisms isolated from a hypersaline cyanobacterial mat. / Abed, Raeid M M; Dobretsov, Sergey; Al-Fori, Marwan; Gunasekera, Sarath P.; Sudesh, Kumar; Paul, Valerie J.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 40, No. 7, 07.2013, p. 759-772.

Research output: Contribution to journalArticle

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AU - Sudesh, Kumar

AU - Paul, Valerie J.

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