Quantitative assessment of total and Gram-positive aerobic bacteria in fresh and ambient-temperature-stored sub-tropical marine fish

Ismail M. Al Bulushi, Susan Poole, Hilton C. Deeth, Gary A. Dykes

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The current study was undertaken to enumerate Gram-positive bacteria in fresh sub-tropical marine fish and determine the effect of ambient storage (25°C) on the Gram-positive bacterial count. Total and Gram-positive bacteria were enumerated in the muscles, gills and gut of fresh and stored Pseudocaranx dentex, Pagrus auratus and Mugil cephalus on tryptone soya agar (TSA) and TSA with 0.25% phenylethyl alcohol (PEA), respectively. Initial studies indicated that PEA significantly reduced total aerobic bacterial count (TABC) whereas control Gram-positive bacteria were not affected by 0.25% PEA. TABC significantly increased in all fish body parts, whereas Gram-positive aerobic bacterial count (GABC) significantly increased only in the muscles and gills during ambient storage for 15 h. The TABC of the fish species increased from 4.00, 6.13 and 4.58 log cfu g-1, respectively in the muscles, gills, and gut to 6.31, 7.31 and 7.23 log cfu g-1 by the end of storage. GABC increased from 2.00, 3.52 and 2.20 log cfu g-1 to 4.70, 5.85 and 3.36 log cfu g-1. Within each species, TABC were significantly higher in the gills compared to that of muscles and gut; however, no significant differences were found in GABC between muscles and gills. This study demonstrated the potential importance of Gram-positive bacteria in sub-tropical marine fish and their spoilage.

Original languageEnglish
Pages (from-to)1867-1875
Number of pages9
JournalWorld Journal of Microbiology and Biotechnology
Volume24
Issue number9
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Aerobic bacteria
tropical fish
Aerobic Bacteria
Bacterial Load
Gram-Positive Bacteria
marine fish
plate count
Fish
Muscle
Phenylethyl Alcohol
ambient temperature
Fishes
Bacteria
Temperature
bacteria
gills
Gram-positive bacteria
Muscles
muscles
Agar

Keywords

  • Ambient temperature
  • Gram-positive bacteria
  • Spoilage
  • Sub-tropical marine fish

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Plant Science
  • Biochemistry
  • Food Science
  • Microbiology
  • Biotechnology

Cite this

Quantitative assessment of total and Gram-positive aerobic bacteria in fresh and ambient-temperature-stored sub-tropical marine fish. / Al Bulushi, Ismail M.; Poole, Susan; Deeth, Hilton C.; Dykes, Gary A.

In: World Journal of Microbiology and Biotechnology, Vol. 24, No. 9, 09.2008, p. 1867-1875.

Research output: Contribution to journalArticle

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