TY - JOUR
T1 - PURIFICATION AND CHARACTERIZATION OF PHENOLOXIDASE ISOFORMS FROM TAIWANESE BLACK TIGER SHRIMP (PENAEUS MONODON)
AU - ROLLE, R. S.
AU - GUIZANI, N.
AU - CHEN, J. S.
AU - MARSHALL, M. R.
AU - YANG, J. S.
AU - WEI, C. I.
PY - 1991/5
Y1 - 1991/5
N2 - Two phenoloxidase (PPO) isoforms were purified from Taiwanese black tiger shrimp (Penaeus monodon) purified via ammonium sulfate precipitation and high performance hydrophobic interaction chromatography on phenyl Sepharose CL‐4B. Optimal DL‐ã‐3, 4‐dihydroxyphenylalanine (D, L‐DOPA) oxidation by these isoforms was observed to occur at pH 6.0, and at 45°C. The isoforms showed both mono‐ and di‐PPO activities, and preferential medabolism of both monoand di‐phenolic substrates lacking a carboxyl group either directly attached to the ring, or in the side chain moiety of the phenolic structure.
AB - Two phenoloxidase (PPO) isoforms were purified from Taiwanese black tiger shrimp (Penaeus monodon) purified via ammonium sulfate precipitation and high performance hydrophobic interaction chromatography on phenyl Sepharose CL‐4B. Optimal DL‐ã‐3, 4‐dihydroxyphenylalanine (D, L‐DOPA) oxidation by these isoforms was observed to occur at pH 6.0, and at 45°C. The isoforms showed both mono‐ and di‐PPO activities, and preferential medabolism of both monoand di‐phenolic substrates lacking a carboxyl group either directly attached to the ring, or in the side chain moiety of the phenolic structure.
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U2 - 10.1111/j.1745-4514.1991.tb00141.x
DO - 10.1111/j.1745-4514.1991.tb00141.x
M3 - Article
AN - SCOPUS:0002719449
SN - 0145-8884
VL - 15
SP - 17
EP - 32
JO - Journal of Food Biochemistry
JF - Journal of Food Biochemistry
IS - 1
ER -