PURIFICATION AND CHARACTERIZATION OF PHENOLOXIDASE ISOFORMS FROM TAIWANESE BLACK TIGER SHRIMP (PENAEUS MONODON)

R. S. ROLLE, N. GUIZANI, J. S. CHEN, M. R. MARSHALL, J. S. YANG, C. I. WEI

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Two phenoloxidase (PPO) isoforms were purified from Taiwanese black tiger shrimp (Penaeus monodon) purified via ammonium sulfate precipitation and high performance hydrophobic interaction chromatography on phenyl Sepharose CL‐4B. Optimal DL‐ã‐3, 4‐dihydroxyphenylalanine (D, L‐DOPA) oxidation by these isoforms was observed to occur at pH 6.0, and at 45°C. The isoforms showed both mono‐ and di‐PPO activities, and preferential medabolism of both monoand di‐phenolic substrates lacking a carboxyl group either directly attached to the ring, or in the side chain moiety of the phenolic structure.

Original languageEnglish
Pages (from-to)17-32
Number of pages16
JournalJournal of Food Biochemistry
Volume15
Issue number1
DOIs
Publication statusPublished - 1991

Fingerprint

Penaeidae
Tigers
Monophenol Monooxygenase
Penaeus monodon
monophenol monooxygenase
ammonium sulfate
agarose
Protein Isoforms
shrimp
oxidation
Ammonium Sulfate
Hydrophobic and Hydrophilic Interactions
Chromatography
hydrophobic interaction chromatography

ASJC Scopus subject areas

  • Food Science
  • Biophysics
  • Pharmacology
  • Cell Biology

Cite this

PURIFICATION AND CHARACTERIZATION OF PHENOLOXIDASE ISOFORMS FROM TAIWANESE BLACK TIGER SHRIMP (PENAEUS MONODON). / ROLLE, R. S.; GUIZANI, N.; CHEN, J. S.; MARSHALL, M. R.; YANG, J. S.; WEI, C. I.

In: Journal of Food Biochemistry, Vol. 15, No. 1, 1991, p. 17-32.

Research output: Contribution to journalArticle

ROLLE, R. S. ; GUIZANI, N. ; CHEN, J. S. ; MARSHALL, M. R. ; YANG, J. S. ; WEI, C. I. / PURIFICATION AND CHARACTERIZATION OF PHENOLOXIDASE ISOFORMS FROM TAIWANESE BLACK TIGER SHRIMP (PENAEUS MONODON). In: Journal of Food Biochemistry. 1991 ; Vol. 15, No. 1. pp. 17-32.
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