Purification and characterization of antimicrobial peptides from the skin secretions of the mink frog (Rana septentrionalis)

Catherine R. Bevier, Agnes Sonnevend, Jolanta Kolodziejek, Norbert Nowotny, Per F. Nielsen, J. Michael Conlon

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Skin secretions were obtained from male, female, and juvenile specimens of the mink frog (Rana septentrionalis) by electric stimulation and shown to contain 10 peptides that differentially inhibited the growth of microorganisms. The elution profiles of secretions from the three groups following reverse-phase HPLC were almost identical indicating that there were no major sexual or developmental differences in chemical composition. Four peptides of the brevinin-1 family, with potent antimicrobial activity and strong hemolytic activity, two members of ranatuerin-2 family and three members of the temporin family, were purified and characterized structurally. A 21-amino-acid C-terminally α-amidated peptide (GIWDTIKSMGKVFAGKILQNL.NH 2) with broad-spectrum antimicrobial activity was also isolated from the skin secretions. This peptide shows limited structural similarity with the N-terminal region of brevinin-2 peptides previously isolated from R. temporaria skin but lacks the C-terminal cyclic heptapeptide domain associated with this family. Molecular and morphological data support the placement of R. septentrionalis in the R. catesbeiana species group, but analysis based upon the distribution of the molecular forms of the antimicrobial peptides is indicative of a closer phylogenetic relationship between R. septentrionalis and the frogs of the R. pipiens and R. boylii groups.

Original languageEnglish
Pages (from-to)31-38
Number of pages8
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume139
Issue number1-3
DOIs
Publication statusPublished - Oct 2004

Fingerprint

Ranidae
Mink
Anura
Purification
Skin
Peptides
Microorganisms
Electric Stimulation
High Pressure Liquid Chromatography
Amino Acids
Growth
Chemical analysis

Keywords

  • Antimicrobial peptide
  • Brevinin-1
  • Frog skin
  • Ranatuerin-2
  • Temporin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Pharmacology

Cite this

Purification and characterization of antimicrobial peptides from the skin secretions of the mink frog (Rana septentrionalis). / Bevier, Catherine R.; Sonnevend, Agnes; Kolodziejek, Jolanta; Nowotny, Norbert; Nielsen, Per F.; Michael Conlon, J.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 139, No. 1-3, 10.2004, p. 31-38.

Research output: Contribution to journalArticle

Bevier, Catherine R. ; Sonnevend, Agnes ; Kolodziejek, Jolanta ; Nowotny, Norbert ; Nielsen, Per F. ; Michael Conlon, J. / Purification and characterization of antimicrobial peptides from the skin secretions of the mink frog (Rana septentrionalis). In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology. 2004 ; Vol. 139, No. 1-3. pp. 31-38.
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