Pseudoalteromonas spongiae sp. nov., a novel member of the γ-proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters

Stanley C K Lau, Mandy M Y Tsoi, Xiancui Li, Sergey Dobretsov, Yulia Plakhotnikova, Po Keung Wong, Pei Yuan Qian

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A Gram-negative, non-spore-forming, short rod-shaped bacterium (UST010723-006T) was isolated from the surface of the sponge Mycale adhaerens in Hong Kong waters. Cells of UST010723-006T did not have flagella and were non-motile. Colonies were pale orange in colour, 2-4 mm in diameter, convex with a smooth surface and an entire translucent margin. Gas bubbles were observed in the colonies and also in the agar matrix underneath and adjacent to the colonies. UST010723-006T was heterotrophic, strictly aerobic and required NaCl for growth (2.0-6.0%). It grew at pH 5.0-10.0 and between 12 and 44 °C. Phylogenetic analysis of the 16S rRNA gene sequence placed UST010723-006T within the genus Pseudoalteromonas of the γ-subclass of the Proteobacteria. The DNA G+C content is 40.6 mol% and the dominant fatty acids were 12:0 3-OH, 14:0, 15:0 iso 2-OH, 16:0, 16:1ω7, 17:1ω8 and 18:1ω7 (altogether representing 75.9% of the total). These data supported the affiliation of UST010723-006T to the genus Pseudoalteromonas. The closest relatives were Pseudoalteromonas luteviolacea, P. phenolica, P. rubra and P. ruthenica with similarity values ranging from 95.4 to 96.8%. UST010723-006T differed from these closest relatives by 9-19 traits. Molecular evidence, together with phenotypic characteristics, suggests that UST010723-006T constitutes a novel species within the genus Pseudoalteromonas. The name Pseudoalteromonas spongiae sp. nov. is proposed for this bacterium. The type strain is UST010723-006T (=NRRL B-41100T=JCM 12884T).

Original languageEnglish
Pages (from-to)1593-1596
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume55
Issue number4
DOIs
Publication statusPublished - Jul 2005

Fingerprint

Pseudoalteromonas
Mycale
Proteobacteria
Porifera
Hong Kong
sponge
China
Water
new species
bacterium
Pseudoalteromonas rubra
Pseudoalteromonas ruthenica
agar
water
bubble
Bacteria
fatty acid
Flagella
phylogenetics
bacteria

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Ecology, Evolution, Behavior and Systematics
  • Immunology

Cite this

Pseudoalteromonas spongiae sp. nov., a novel member of the γ-proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters. / Lau, Stanley C K; Tsoi, Mandy M Y; Li, Xiancui; Dobretsov, Sergey; Plakhotnikova, Yulia; Wong, Po Keung; Qian, Pei Yuan.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 55, No. 4, 07.2005, p. 1593-1596.

Research output: Contribution to journalArticle

Lau, Stanley C K ; Tsoi, Mandy M Y ; Li, Xiancui ; Dobretsov, Sergey ; Plakhotnikova, Yulia ; Wong, Po Keung ; Qian, Pei Yuan. / Pseudoalteromonas spongiae sp. nov., a novel member of the γ-proteobacteria isolated from the sponge Mycale adhaerens in Hong Kong waters. In: International Journal of Systematic and Evolutionary Microbiology. 2005 ; Vol. 55, No. 4. pp. 1593-1596.
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AU - Dobretsov, Sergey

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AU - Qian, Pei Yuan

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