Protective effects of emodin against cisplatin-induced oxidative stress in cultured human kidney (HEK 293) cells

Mostafa I. Waly, Badreldin H. Ali, Intisar Al-Lawati, Abderrahim Nemmar

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Emodin (a rhubarb anthraquinone) has strong antioxidant and anticancer actions, and recent studies indicated that it reduces cellular oxidative stress induced by various insults and drugs. Cisplatin is an anticancer drug that is associated with nephrotoxicity and induces oxidative stress in cultured human kidney (HEK 293) cells. This study aimed to assess the in-vitro antioxidant properties of the emodin against cisplatin-induced oxidative stress in HEK 293 cells. Our study revealed that emodin acted as a potent free radical scavenger and provided nephroprotection against cisplatin-induced oxidative stress. Emodin as low as 0.5μm did not decrease cell viability and restored the cisplatin-induced glutathione depletion and total antioxidant capacity in a dose-dependent manner. Emodin augmented the cisplatin-induced inhibition of antioxidant enzymes (catalase, glutathione peroxidase, glutathione S-transferase, glutathione reductase and superoxide dismutase). These results suggest that emodin has the potential to be used as an adjunct therapeutic agent in patients receiving cisplatin treatment.

Original languageEnglish
Pages (from-to)626-630
Number of pages5
JournalJournal of Applied Toxicology
Volume33
Issue number7
DOIs
Publication statusPublished - Jul 2013

Keywords

  • Antioxidants
  • Cisplatin
  • Emodin
  • Oxidative stress

ASJC Scopus subject areas

  • Toxicology

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