Prolactin receptor immunoreactivity in rat anterior pituitary

Gérard Morel, Allal Ouhtit, Paul A. Kelly

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Prolactin (PRL) receptors have been identified in both classical and nonclassi- cal target organs. Actions of PRL on the secretion of anterior pituitary hormones have been reported. In order to confirm the presence of PRL receptors on specific cell types of the anterior pituitary, immunocytology on ultrathin frozen sections was carried out using monoclonal antibodies specific to the rat PRL receptor. Anterior pituitary glands were removed and fixed in 4% paraformaldehyde or 2.5% glutaraldehyde and postfixed in 1% osmium tetroxide. Tissues were frozen and ultrathin sections were made by cryoultramicrotomy. In anterior pituitary cells, all endocrine cell populations showed PRL-R-like immunoreactivity. The relative labeling frequency based on immunolabeling studies was somatotrophs > lactotrophs > thyrotrophs ∼ corticotrophs > gonadotrophs. Within all endocrine cell types, no apparent differences could be observed in the subcellular localization of PRL-R-like immunoreactivity. At the subcellular level, gold particles, either isolated or in groups of two, were seen at the level of the plasma membrane, in the cytoplasmic matrix, in the vicinity of the endoplasmic reticulum and in the nucleus. The identification of receptors specific to PRL within different cells of the anterior pituitary raises the interesting possibility that PRL may act via an autocrine or paracrine network in regulating anterior pituitary function.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalNeuroendocrinology
Volume59
Issue number1
DOIs
Publication statusPublished - 1994

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Keywords

  • Anterior pituitary
  • Corticotroph
  • Electron microscopy
  • Gonadotroph
  • Immunocytology
  • Lactotroph
  • PRL receptor
  • Somatotroph
  • Thyrotroph

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Cellular and Molecular Neuroscience
  • Endocrine and Autonomic Systems
  • Neuroscience(all)

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