TY - JOUR
T1 - Prolactin receptor expression by lymphoid tissues in normal and immunized rats
AU - Koh, Crystal Y.
AU - Phillips, J. Theodore
N1 - Funding Information:
The authors wish to thank Dr. Paul A. Kelly for generouslyp rovidinga nti-prolactinr eceptora ntibodies for theses tudiesa nd Ms. Cheryl Beisert for assistance with preparation of the manuscript.T his work was supportedb y NIH grant R29-DK39526.
PY - 1993/4
Y1 - 1993/4
N2 - Prolactin receptor (PRLr) expression and distribution in thymus, spleen, bone marrow, lymph nodes, and peripheral blood lymphocytes from young adult Lewis rats are analyzed using single-color flow cytometry and a well-characterized monoclonal antibody directed against the rat liver PRLr. The in vivo effects of regional immunization on PRLr expression are also examined. PRLr is found to be widely distributed among cells of the immune system and demonstrates lymphoid tissue-specific patterns of expression. Footpad immunization caused the rapid, but transient, induction of PRLr expression in the draining lymph node, with only modest effects on PRLr expression in other distant lymphoid tissues. These studies indicate that PRL may be capable of direct interaction with the immune system through differential expression of the PRL cell surface receptor on select lymphoid target cell populations.
AB - Prolactin receptor (PRLr) expression and distribution in thymus, spleen, bone marrow, lymph nodes, and peripheral blood lymphocytes from young adult Lewis rats are analyzed using single-color flow cytometry and a well-characterized monoclonal antibody directed against the rat liver PRLr. The in vivo effects of regional immunization on PRLr expression are also examined. PRLr is found to be widely distributed among cells of the immune system and demonstrates lymphoid tissue-specific patterns of expression. Footpad immunization caused the rapid, but transient, induction of PRLr expression in the draining lymph node, with only modest effects on PRLr expression in other distant lymphoid tissues. These studies indicate that PRL may be capable of direct interaction with the immune system through differential expression of the PRL cell surface receptor on select lymphoid target cell populations.
KW - (Rat)
KW - Anti-receptor antibody
KW - Flow cytometry
KW - Lymphocyte
KW - Prolactin receptor
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U2 - 10.1016/0303-7207(93)90021-B
DO - 10.1016/0303-7207(93)90021-B
M3 - Article
C2 - 8319823
AN - SCOPUS:0027286033
SN - 0303-7207
VL - 92
SP - R21-R25
JO - Molecular and Cellular Endocrinology
JF - Molecular and Cellular Endocrinology
IS - 2
ER -