Production of thermotolerant, detergent stable alkaline protease using the gut waste of Sardinella longiceps as a substrate

Optimization and characterization

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The gut wastes of Sardinella longiceps were used as substrate for protease production. The gut waste has 61.6% proteins, 21.8% lipids, 8.5% carbohydrates on dry weight basis and trace elements. The significant factors of protease fermentation were screened by Plackett-Burman design. A protease activity of 68.56 U/ml was predicted at 46.31 °C, incubation time 71.11 h, inoculum 4.86% (v/v) and substrate concentration 2.66% (w/v), using response surface methodology. However, the validation experiment showed 73.52 U/ml activity. The artificial neural network was found as a better tool to predict the experimental results. The partially purified protease showed higher activity at pH 9 and 10 and retained 90% activity after 120 h at pH 9. It showed maximum activity at 50 °C and retained 88% residual activity until 90 min at 50 °C. Zn++ enhanced the protease activity by 40%. The protease retained an activity of 93, 103, 90 and 98% against urea, β-mercaptoethanol, SDS and tween 80 respectively. The alkaline protease was compatible with all the commercial detergents tested with the residual activity above 90%. The alkaline protease exhibited 22% higher activity on the tryptone soya substrate. The gut waste of S. longiceps is a worthy low cost substrate for the production of industrially important alkaline protease.

Original languageEnglish
Article number12442
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - Dec 1 2018

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detergents
proteinases
digestive system
Sardinella longiceps
response surface methodology
neural networks
trace elements
inoculum
urea
fermentation
carbohydrates
lipids

ASJC Scopus subject areas

  • General

Cite this

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title = "Production of thermotolerant, detergent stable alkaline protease using the gut waste of Sardinella longiceps as a substrate: Optimization and characterization",
abstract = "The gut wastes of Sardinella longiceps were used as substrate for protease production. The gut waste has 61.6{\%} proteins, 21.8{\%} lipids, 8.5{\%} carbohydrates on dry weight basis and trace elements. The significant factors of protease fermentation were screened by Plackett-Burman design. A protease activity of 68.56 U/ml was predicted at 46.31 °C, incubation time 71.11 h, inoculum 4.86{\%} (v/v) and substrate concentration 2.66{\%} (w/v), using response surface methodology. However, the validation experiment showed 73.52 U/ml activity. The artificial neural network was found as a better tool to predict the experimental results. The partially purified protease showed higher activity at pH 9 and 10 and retained 90{\%} activity after 120 h at pH 9. It showed maximum activity at 50 °C and retained 88{\%} residual activity until 90 min at 50 °C. Zn++ enhanced the protease activity by 40{\%}. The protease retained an activity of 93, 103, 90 and 98{\%} against urea, β-mercaptoethanol, SDS and tween 80 respectively. The alkaline protease was compatible with all the commercial detergents tested with the residual activity above 90{\%}. The alkaline protease exhibited 22{\%} higher activity on the tryptone soya substrate. The gut waste of S. longiceps is a worthy low cost substrate for the production of industrially important alkaline protease.",
author = "Aishwarya Ramkumar and Sivakumar Nallusamy and Ashish Gujrathi and Reginald Victor",
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