Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B

Sanket Joshi, Chirag Bharucha, Anjana J. Desai

Research output: Contribution to journalArticle

145 Citations (Scopus)

Abstract

A biosurfactant producing strain, Bacillus subtilis 20B, was isolated from fermented food in India. The strain also showed inhibition of various fungi in in-vitro experiments on Potato Dextrose Agar medium. It was capable of growth at temperature 55 °C and salts up to 7%. It utilized different sugars, alcohols, hydrocarbons and oil as a carbon source, with preference for sugars. In glucose based minimal medium it produced biosurfactant which reduced surface tension to 29.5 mN/m, interfacial tension to 4.5 mN/m and gave stable emulsion with crude oil and n-hexadecane. The biosurfactant activity was stable at high temperature, a wide range of pH and salt concentrations for five days. Oil displacement experiments using biosurfactant containing broth in sand pack columns with crude oil showed 30.22% recovery. The possible application of organism as biocontrol agent and use of biosurfactant in microbial enhanced oil recovery (MEOR) is discussed.

Original languageEnglish
Pages (from-to)4603-4608
Number of pages6
JournalBioresource Technology
Volume99
Issue number11
DOIs
Publication statusPublished - Jul 2008

Fingerprint

biosurfactants
Surface Tension
fermented foods
Petroleum
Bacilli
Bacillus subtilis
crude oil
Surface tension
sugar
Oils
Salts
Biocontrol
salt
Sugar Alcohols
Glucose
Recovery
Food
Mineral Oil
oils
Temperature

Keywords

  • Bacillus subtilis
  • Biosurfactant
  • Interfacial tension
  • MEOR, Biocontrol agent
  • Surface tension

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Food Science
  • Process Chemistry and Technology
  • Applied Microbiology and Biotechnology

Cite this

Production of biosurfactant and antifungal compound by fermented food isolate Bacillus subtilis 20B. / Joshi, Sanket; Bharucha, Chirag; Desai, Anjana J.

In: Bioresource Technology, Vol. 99, No. 11, 07.2008, p. 4603-4608.

Research output: Contribution to journalArticle

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