Production of bioplastic (poly-3-hydroxybutyrate) using waste paper as a feedstock: Optimization of enzymatic hydrolysis and fermentation employing Burkholderia sacchari

Huda Al-Battashi, Neelamegam Annamalai, Shatha Al-Kindi, Anu Sadasivan Nair, Saif Al-Bahry, Jay Prakash Verma, Sivakumar Nallusamy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

The global demand for bio-plastic particularly polyhydroxyalkanoate (PHA) have been increased in the last few decades as a substitute of petrochemical-based plastic. Utilization of waste paper, the primary constituent of municipal solid waste (MSW), as a carbon source for polyhydroxybutyrate (PHB) production is not only an alternative, environmental friendly route of waste management but also helps to valorize the waste. In this study, hydrogen peroxide pretreated waste paper saccharification has been optimized using central composite design (CCD). The maximum hydrolysis (88.18%) occurred at paper loading 5.0 g/L, agitation 242 rpm, working volume 20%, cellulase 49.82 U/g, β-glucosidase 20.9 U/g and hemicellulase 29.5 U/g. PHB synthesis and biomass accumulation by xylose-utilizing Burkholderia sacchari using waste paper hydrolysate were studied using different nitrogen sources and carbon to nitrogen (C/N) ratios. Maximum PHB and dry cell weight (DCW) occurred with ammonium sulfate and a C/N ratio of 20. The highest biomass (3.63 g/L), the maximum PHB accumulation (44.2%) and the maximum reducing sugar utilization (92.1%) were observed after 48 h of cultivation using diluted hydrolysate. The physicochemical properties of the extracted PHB were compatible with the standard PHB. Hence, the waste paper could be exploited as a renewable feedstock for the sustainable production of PHB.

Original languageEnglish
Pages (from-to)236-247
Number of pages12
JournalJournal of Cleaner Production
Volume214
DOIs
Publication statusPublished - Mar 20 2019

Keywords

  • Burkholderia sacchari
  • Hydrogen peroxide pretreatment
  • Optimization
  • Poly-3-hydroxybutyrate
  • Saccharification
  • Waste paper

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Environmental Science(all)
  • Strategy and Management
  • Industrial and Manufacturing Engineering

Fingerprint Dive into the research topics of 'Production of bioplastic (poly-3-hydroxybutyrate) using waste paper as a feedstock: Optimization of enzymatic hydrolysis and fermentation employing Burkholderia sacchari'. Together they form a unique fingerprint.

Cite this