Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis

Viviane Benetka, Anna Kübber-Heiss, Jolanta Kolodziejek, Norbert Nowotny, Margarete Hofmann-Parisot, Karin Möstl

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Feline coronaviruses (FCoV) vary widely in virulence causing a spectrum of clinical manifestations reaching from subclinical course to fatal feline infectious peritonitis (FIP). Independent of virulence variations they are separated into two different types, type I, the original FCoV, and type II, which is closely related to canine coronavirus (CCV). The prevalence of FCoV types in Austrian cat populations without FIP has been surveyed recently indicating that type I infections predominate. The distribution of FCoV types in cats, which had succumbed to FIP, however, was fairly unknown. PCR assays have been developed amplifying parts of the spike protein gene. Type-specific primer pairs were designed, generating PCR products of different sizes. A total of 94 organ pools of cats with histopathologically verified FIP was tested. A clear differentiation was achieved in 74 cats, 86% of them were type I positive, 7% type II positive, and 7% were positive for both types. These findings demonstrate that in FIP cases FCoV type I predominates, too, nonetheless, in 14% of the cases FCoV type II was detected, suggesting its causative involvement in cases of FIP.

Original languageEnglish
Pages (from-to)31-42
Number of pages12
JournalVeterinary Microbiology
Volume99
Issue number1
DOIs
Publication statusPublished - Mar 26 2004

Fingerprint

Feline Infectious Peritonitis
Feline Coronavirus
Feline coronavirus
feline infectious peritonitis
Cats
cats
Virulence
Canine Coronavirus
virulence
Canine coronavirus
Polymerase Chain Reaction
assays
Infection

Keywords

  • Feline coronavirus (FCoV)
  • Feline infectious peritonitis (FIP)
  • Paraffin embedded tissues (PET)
  • RT-PCR
  • Types I and II

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Microbiology
  • veterinary(all)

Cite this

Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis. / Benetka, Viviane; Kübber-Heiss, Anna; Kolodziejek, Jolanta; Nowotny, Norbert; Hofmann-Parisot, Margarete; Möstl, Karin.

In: Veterinary Microbiology, Vol. 99, No. 1, 26.03.2004, p. 31-42.

Research output: Contribution to journalArticle

Benetka, Viviane ; Kübber-Heiss, Anna ; Kolodziejek, Jolanta ; Nowotny, Norbert ; Hofmann-Parisot, Margarete ; Möstl, Karin. / Prevalence of feline coronavirus types I and II in cats with histopathologically verified feline infectious peritonitis. In: Veterinary Microbiology. 2004 ; Vol. 99, No. 1. pp. 31-42.
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