Power generation from cellulose using mixed and pure cultures of cellulose-degrading bacteria in a microbial fuel cell

Sedky H.A. Hassan, Yong Seong Kim, Sang Eun Oh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

88 Citations (Scopus)

Abstract

Microbial fuel cells (MFCs) have been used to generate electricity from various organic compounds such as acetate, glucose, and lactate. We demonstrate here that electricity can be produced in an MFC using cellulose as the electron donor source. Tests were conducted using two-chambered MFCs, the anode medium was inoculated with mixed or pure culture of cellulose-degrading bacteria Nocardiopsis sp. KNU (S strain) or Streptomyces enissocaesilis KNU (K strain), and the catholyte in the cathode compartment was 50mM ferricyanide as catholyte. The power density for the mixed culture was 0.188mW (188mW/m2) at a current of 0.5mA when 1g/L cellulose was used. However, the power density decreased as the cellulose concentration in the anode compartment decreased. The columbic efficiencies (CEs) ranged from 41.5 to 33.4%, corresponding to an initial cellulose concentration of 0.1-1.0g/L. For the pure culture, cellobioase enzyme was added to increase the conversion of cellulose to simple sugars, since electricity production is very low. The power densities for S and K strain pure cultures with cellobioase were 162mW/m2 and 145mW/m2, respectively. Cyclic voltammetry (CV) experiments showed the presence of peaks at 380, 500, and 720mV vs. Ag/AgCl for the mixed bacterial culture, indicating its electrochemical activity without an external mediator. Furthermore, this MFC system employs a unique microbial ecology in which both the electron donor (cellulose) and the electron acceptor (carbon paper) are insoluble.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalEnzyme and Microbial Technology
Volume51
Issue number5
DOIs
Publication statusPublished - Oct 10 2012
Externally publishedYes

Keywords

  • Cellobioase
  • Cellulose
  • Electricity generation
  • Microbial fuel cells
  • Mixed bacterial culture

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Fingerprint

Dive into the research topics of 'Power generation from cellulose using mixed and pure cultures of cellulose-degrading bacteria in a microbial fuel cell'. Together they form a unique fingerprint.

Cite this