Potential of citrus budlings originating in the Middle East as sources of citrus viroids

S. A. Al-Harthi, A. M. Al-Sadi, A. A. Al-Saady

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A study was conducted to determine the potential of citrus budlings originating in the Middle East as sources of citrus viroids. A total of 101 citrus budlings belonging to 9 citrus cultivars which originated from 5 countries were collected from commercial nurseries in 2010. A multiplex reverse transcription polymerase chain reaction (mRT-PCR) was used for detection of CEVd, CBLVd, HSVd, CDVd and CBCVd viroids from the budlings. Multiplex RT-PCR analysis, followed by sequencing of representative isolates, showed that 29, 28, 54, 68 and 79% of the citrus budlings are positive for CEVd, CBLVd, HSVd, CDVd and CBCVd viroids, respectively. Viroids were detected in citrus budlings originating in Oman, Lebanon, Syria, Egypt and Jordon. Viroids from Oman were found to share 94-100% nucleotide similarity with viroids from other parts of the world. Findings from this study provide evidence for circulation of citrus viroids via budlings originating in the Middle East. Strict quarantine measures and certification programs are required to reduce widespread distribution of citrus viroids via citrus budlings originating in these countries.

Original languageEnglish
Pages (from-to)13-15
Number of pages3
JournalCrop Protection
Volume48
DOIs
Publication statusPublished - Jun 2013

Fingerprint

viroids
Middle East
Citrus
Citrus bent leaf viroid
Oman
reverse transcriptase polymerase chain reaction
Lebanon
Syria
certification
quarantine
Egypt
nucleotides

Keywords

  • Acid lime
  • Exocortis

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Potential of citrus budlings originating in the Middle East as sources of citrus viroids. / Al-Harthi, S. A.; Al-Sadi, A. M.; Al-Saady, A. A.

In: Crop Protection, Vol. 48, 06.2013, p. 13-15.

Research output: Contribution to journalArticle

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