Abstract
Abiotic stresses in plants such as salinity, drought, heavy metal toxicity, heat, and nutrients limitations significantly reduce agricultural production worldwide. The genome editing techniques such as transcriptional activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs) have been used for genome manipulations in plants. However, clustered regularly inters-paced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) technique has recently emerged as a promising tool for genome editing in plants to acquire desirable traits. The CRISPR/Cas9 system has a great potential to develop crop varieties with improved tolerance against abiotic stresses. This review is centered on the biology and potential application of the CRISPR/Cas9 system to improve abiotic stress tolerance in plants. Furthermore, this review high-lighted the recent advancements of CRISPR/Cas9-mediated genome editing for sustainable agriculture.
Original language | English |
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Pages (from-to) | 861-877 |
Number of pages | 17 |
Journal | Protein and Peptide Letters |
Volume | 28 |
Issue number | 8 |
DOIs | |
Publication status | Published - Aug 2021 |
Keywords
- Abiotic stresses
- CRISPR/Cas9
- Crop improvement
- Genome editing
- SgRNA
- Transcription
ASJC Scopus subject areas
- Structural Biology
- Biochemistry