Possible protective effect of diacerein on doxorubicin-induced nephrotoxicity in rats

Marwa M.M. Refaie, Entesar F. Amin, Nashwa F. El-Tahawy, Aly M. Abdelrahman

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Nephrotoxicity is one of the limiting factors for using doxorubicin (DOX). Interleukin 1 has major role in DOX-induced nephrotoxicity, so we investigated the effect of interleukin 1 receptor antagonist diacerein (DIA) on DOX-induced nephrotoxicity. DIA (25 and 50 mg/kg/day) was administered orally to rats for 15 days, in the presence or absence of nephrotoxicity induced by a single intraperitoneal injection of DOX (15 mg/kg) at the 11th day. We measured levels of serum urea, creatinine, renal reduced glutathione (GSH), malondialdehyde (MDA), total nitrites (NOx), catalase, and superoxide dismutase (SOD). In addition, caspase-3, tumor necrosis factor alpha (TNFα), nuclear factor kappa B (NFB) expressions, and renal histopathology were assessed. Our results showed that DOX-induced nephrotoxicity was ameliorated or reduced by both doses of DIA, but diacerein high dose (DHD) showed more improvement than diacerein low dose (DLD). This protective effect was manifested by significant improvement in all measured parameters compared to DOX treated group by using DHD. DLD showed significant improvement of creatinine, MDA, NOx, GSH, histopathology, and immunohistochemical parameters compared to DOX treated group.

Original languageEnglish
Article number9507563
JournalJournal of Toxicology
Volume2016
DOIs
Publication statusPublished - 2016

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diacetylrhein
Doxorubicin
Rats
Malondialdehyde
Creatinine
Kidney
Interleukin-1 Receptors
NF-kappa B
Nitrites
Intraperitoneal Injections
Interleukin-1
Caspase 3
Catalase
Superoxide Dismutase
Glutathione
Urea

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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Possible protective effect of diacerein on doxorubicin-induced nephrotoxicity in rats. / Refaie, Marwa M.M.; Amin, Entesar F.; El-Tahawy, Nashwa F.; Abdelrahman, Aly M.

In: Journal of Toxicology, Vol. 2016, 9507563, 2016.

Research output: Contribution to journalArticle

Refaie, Marwa M.M. ; Amin, Entesar F. ; El-Tahawy, Nashwa F. ; Abdelrahman, Aly M. / Possible protective effect of diacerein on doxorubicin-induced nephrotoxicity in rats. In: Journal of Toxicology. 2016 ; Vol. 2016.
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