Population structure of Stemphylium lycopersici associated with leaf spot of tomato in a single field

Karima Al-Amri, Abdullah M. Al-Sadi, Adel Al-Shihi, Abbas Nasehi, Issa Al-Mahmooli, Mike L. Deadman

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Stemphylium lycopersici is an important pathogen causing leaf spot of tomatoes worldwide. Although much information is available about the pathogen, little is known about dynamics of S. lycopersici in tomato fields. Seventy-nine symptomatic leaf samples were collected from two tomato cultivars grown in a farm (Miral and Inbred line). Fungal species associated with the disease were isolated on potato dextrose agar. Seventy-nine isolates were obtained and identified as S. lycopersici based on sequence analysis of combined dataset of the internal transcribed spacer and glyceraldehyde-3-phosphate dehydrogenase regions. The 79 isolates were subjected to amplified fragment length polymorphism analysis using three primer combinations. The Stemphylium lycopersici population from the two cultivars was found to have a very low level of genetic diversity (H = 0.0948). Cluster analysis showed intermixing of isolates from the two cultivars. In addition, analysis of molecular variance showed the presence of a very low level of genetic differentiation between populations obtained from the two cultivars (Fst = 0.0206). These findings indicate the presence of a high rate of gene flow between the two populations and may suggest that the two populations originated from the same inoculum source. The implications of these findings on the management of Stemphylium-induced leaf spot of tomatoes are discussed.

Original languageEnglish
Article number1642
JournalSpringerPlus
Volume5
Issue number1
DOIs
Publication statusPublished - Dec 1 2016

Fingerprint

leaf spot
Stemphylium
population structure
tomatoes
cultivars
genetic variation
glyceraldehyde-3-phosphate dehydrogenase
pathogens
inbred lines
internal transcribed spacers
amplified fragment length polymorphism
cluster analysis
gene flow
inoculum
sequence analysis
agar
potatoes
farms
glucose
leaves

Keywords

  • gpd
  • ITS
  • Phylogeny
  • Population structure
  • Solanum lycopersicum

ASJC Scopus subject areas

  • General

Cite this

Population structure of Stemphylium lycopersici associated with leaf spot of tomato in a single field. / Al-Amri, Karima; Al-Sadi, Abdullah M.; Al-Shihi, Adel; Nasehi, Abbas; Al-Mahmooli, Issa; Deadman, Mike L.

In: SpringerPlus, Vol. 5, No. 1, 1642, 01.12.2016.

Research output: Contribution to journalArticle

Al-Amri, Karima ; Al-Sadi, Abdullah M. ; Al-Shihi, Adel ; Nasehi, Abbas ; Al-Mahmooli, Issa ; Deadman, Mike L. / Population structure of Stemphylium lycopersici associated with leaf spot of tomato in a single field. In: SpringerPlus. 2016 ; Vol. 5, No. 1.
@article{417e7033976546a28f79d2a5742bbf44,
title = "Population structure of Stemphylium lycopersici associated with leaf spot of tomato in a single field",
abstract = "Stemphylium lycopersici is an important pathogen causing leaf spot of tomatoes worldwide. Although much information is available about the pathogen, little is known about dynamics of S. lycopersici in tomato fields. Seventy-nine symptomatic leaf samples were collected from two tomato cultivars grown in a farm (Miral and Inbred line). Fungal species associated with the disease were isolated on potato dextrose agar. Seventy-nine isolates were obtained and identified as S. lycopersici based on sequence analysis of combined dataset of the internal transcribed spacer and glyceraldehyde-3-phosphate dehydrogenase regions. The 79 isolates were subjected to amplified fragment length polymorphism analysis using three primer combinations. The Stemphylium lycopersici population from the two cultivars was found to have a very low level of genetic diversity (H = 0.0948). Cluster analysis showed intermixing of isolates from the two cultivars. In addition, analysis of molecular variance showed the presence of a very low level of genetic differentiation between populations obtained from the two cultivars (Fst = 0.0206). These findings indicate the presence of a high rate of gene flow between the two populations and may suggest that the two populations originated from the same inoculum source. The implications of these findings on the management of Stemphylium-induced leaf spot of tomatoes are discussed.",
keywords = "gpd, ITS, Phylogeny, Population structure, Solanum lycopersicum",
author = "Karima Al-Amri and Al-Sadi, {Abdullah M.} and Adel Al-Shihi and Abbas Nasehi and Issa Al-Mahmooli and Deadman, {Mike L.}",
year = "2016",
month = "12",
day = "1",
doi = "10.1186/s40064-016-3324-9",
language = "English",
volume = "5",
journal = "SpringerPlus",
issn = "2193-1801",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "1",

}

TY - JOUR

T1 - Population structure of Stemphylium lycopersici associated with leaf spot of tomato in a single field

AU - Al-Amri, Karima

AU - Al-Sadi, Abdullah M.

AU - Al-Shihi, Adel

AU - Nasehi, Abbas

AU - Al-Mahmooli, Issa

AU - Deadman, Mike L.

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Stemphylium lycopersici is an important pathogen causing leaf spot of tomatoes worldwide. Although much information is available about the pathogen, little is known about dynamics of S. lycopersici in tomato fields. Seventy-nine symptomatic leaf samples were collected from two tomato cultivars grown in a farm (Miral and Inbred line). Fungal species associated with the disease were isolated on potato dextrose agar. Seventy-nine isolates were obtained and identified as S. lycopersici based on sequence analysis of combined dataset of the internal transcribed spacer and glyceraldehyde-3-phosphate dehydrogenase regions. The 79 isolates were subjected to amplified fragment length polymorphism analysis using three primer combinations. The Stemphylium lycopersici population from the two cultivars was found to have a very low level of genetic diversity (H = 0.0948). Cluster analysis showed intermixing of isolates from the two cultivars. In addition, analysis of molecular variance showed the presence of a very low level of genetic differentiation between populations obtained from the two cultivars (Fst = 0.0206). These findings indicate the presence of a high rate of gene flow between the two populations and may suggest that the two populations originated from the same inoculum source. The implications of these findings on the management of Stemphylium-induced leaf spot of tomatoes are discussed.

AB - Stemphylium lycopersici is an important pathogen causing leaf spot of tomatoes worldwide. Although much information is available about the pathogen, little is known about dynamics of S. lycopersici in tomato fields. Seventy-nine symptomatic leaf samples were collected from two tomato cultivars grown in a farm (Miral and Inbred line). Fungal species associated with the disease were isolated on potato dextrose agar. Seventy-nine isolates were obtained and identified as S. lycopersici based on sequence analysis of combined dataset of the internal transcribed spacer and glyceraldehyde-3-phosphate dehydrogenase regions. The 79 isolates were subjected to amplified fragment length polymorphism analysis using three primer combinations. The Stemphylium lycopersici population from the two cultivars was found to have a very low level of genetic diversity (H = 0.0948). Cluster analysis showed intermixing of isolates from the two cultivars. In addition, analysis of molecular variance showed the presence of a very low level of genetic differentiation between populations obtained from the two cultivars (Fst = 0.0206). These findings indicate the presence of a high rate of gene flow between the two populations and may suggest that the two populations originated from the same inoculum source. The implications of these findings on the management of Stemphylium-induced leaf spot of tomatoes are discussed.

KW - gpd

KW - ITS

KW - Phylogeny

KW - Population structure

KW - Solanum lycopersicum

UR - http://www.scopus.com/inward/record.url?scp=84988649847&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84988649847&partnerID=8YFLogxK

U2 - 10.1186/s40064-016-3324-9

DO - 10.1186/s40064-016-3324-9

M3 - Article

AN - SCOPUS:84988649847

VL - 5

JO - SpringerPlus

JF - SpringerPlus

SN - 2193-1801

IS - 1

M1 - 1642

ER -