TY - JOUR
T1 - Population Structure and Development of Resistance to Hymexazol Among Fusarium solani Populations from Date Palm, Citrus and Cucumber
AU - Al-Sadi, Abdullah M.
AU - Al-Masoodi, Ruqaya S.
AU - Al-Ismaili, Majid
AU - Al-Mahmooli, Issa H.
N1 - Publisher Copyright:
© 2015 Blackwell Verlag GmbH.
PY - 2015/12
Y1 - 2015/12
N2 - A study was conducted to investigate genetic diversity and sensitivity to hymexazol among 80 isolates of Fusarium solani complex obtained from date palm (30), citrus (31) and cucumber (19). Characterization based on sequences of the EF1α and ITS rRNA showed that isolates belong to F. solani complex MLST type 3 + 4. AFLP analysis produced 980 polymorphic loci, 80 AFLP genotypes and moderate levels of genetic diversity (H = 0.2494). Clustering of the isolates was not related to the host or the geographical origin of the isolates. Analysis of molecular variance (amova) indicated the existence of a low level of genetic differentiation among populations obtained from different hosts (Fst = 0.0162) and regions (Fst = 0.0066). This may provide evidence for frequent movement of inoculum among hosts and regions in Oman, which could be attributed to cultural practices employed by farmers. Isolates of F. solani displayed variation in sensitivity to hymexazol, with EC50 values ranging from 2 to 5745 μg/ml (mean = 878 μg/ml); 19% of the isolates have an EC50 value of more than 1000 μg/ml. Findings are discussed in terms of the factors that affect diversity in F. solani isolates. The study reports for the first time the development of resistance to hymexazol among F. solani isolates from date palm, citrus and cucumber.
AB - A study was conducted to investigate genetic diversity and sensitivity to hymexazol among 80 isolates of Fusarium solani complex obtained from date palm (30), citrus (31) and cucumber (19). Characterization based on sequences of the EF1α and ITS rRNA showed that isolates belong to F. solani complex MLST type 3 + 4. AFLP analysis produced 980 polymorphic loci, 80 AFLP genotypes and moderate levels of genetic diversity (H = 0.2494). Clustering of the isolates was not related to the host or the geographical origin of the isolates. Analysis of molecular variance (amova) indicated the existence of a low level of genetic differentiation among populations obtained from different hosts (Fst = 0.0162) and regions (Fst = 0.0066). This may provide evidence for frequent movement of inoculum among hosts and regions in Oman, which could be attributed to cultural practices employed by farmers. Isolates of F. solani displayed variation in sensitivity to hymexazol, with EC50 values ranging from 2 to 5745 μg/ml (mean = 878 μg/ml); 19% of the isolates have an EC50 value of more than 1000 μg/ml. Findings are discussed in terms of the factors that affect diversity in F. solani isolates. The study reports for the first time the development of resistance to hymexazol among F. solani isolates from date palm, citrus and cucumber.
KW - AFLP fingerprinting
KW - Fungicide resistance
KW - Phylogenetic analysis
UR - http://www.scopus.com/inward/record.url?scp=84945448574&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84945448574&partnerID=8YFLogxK
U2 - 10.1111/jph.12397
DO - 10.1111/jph.12397
M3 - Article
AN - SCOPUS:84945448574
SN - 0931-1785
VL - 163
SP - 947
EP - 955
JO - Journal of Phytopathology
JF - Journal of Phytopathology
IS - 11-12
ER -