Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE

A. M. Al-Sadi, A. N. Al-Wehaibi, R. M. Al-Shariqi, M. S. Al-Hammadi, I. A. Al-Hosni, I. H. Al-Mahmooli, A. G. Al-Ghaithi

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Lasiodiplodia is a common pathogen causing dieback, gummosis, or root necrosis on the three most important fruit crops in Oman and the United Arab Emirates (UAE): date palm (Phoenix dactylifera), Citrus spp., and mango (Mangifera indica). A study was conducted to examine diversity in 64 Lasiodiplodia isolates infecting date palm (24), Citrus (11), and mango (29) in Oman and the UAE. Identification based on sequences of the internal transcribed spacer (ITS) rDNA and EF1α gene showed that date palm isolates belonged to L. hormozganensis (75% of isolates) and L. theobromae (25%); Citrus isolates belonged to L. hormozganensis (45%), L. theobromae (45%), and L. iraniensis (10%); and mango isolates belonged to L. theobromae (59%), L. iraniensis (34%), and L. hormozganensis (7%). Amplified fragment length polymorphism (AFLP) fingerprinting of the 64 isolates using four primer pair combinations produced 64 genotypes and 972 polymorphic alleles. Cluster analysis separated the isolates into four clusters representing the three species. A higher level of genetic diversity was observed in L. iraniensis (0.3105) compared to L. hormozganensis (0.2503) and L. theobromae (0.2331) in Oman. Analysis of molecular variance (AMOVA) indicated the existence of low levels of genetic differentiation among date palm populations of L. hormozganensis obtained from Oman and the UAE (FST = 0.025) and among populations of L. hormozganensis (0.0485) and L. theobromae (0.0703) from date palm, Citrus, and mango. These findings imply a high rate of movement of L. hormozganensis and L. theobromae isolates among date palm, Citrus, and mango and between the two countries. Findings from the pathogenicity test supported the AMOVA analysis and suggested a lack of host specialization in L. hormozganensis, L. iraniensis, and L. theobromae on date palm, acid lime, and mango. Although this is the first record of L. hormozganensis and L. iraniensis in Oman, the relatively moderate level of genetic diversity in the two species compared to L. theobromae suggests that the two species have been in Oman for a long time but misidentified by morphology and ITS rDNA sequences as L. theobromae. This study is also the first record of date palm and acid lime as natural hosts for L. hormozganensis and the first record of L. hormozganensis in the UAE. The diversity in Lasiodiplodia species affecting date palm, Citrus, and mango in Oman and the UAE should be taken into consideration when planning future management programs for diseases caused by these pathogens.

Original languageEnglish
Pages (from-to)1363-1369
Number of pages7
JournalPlant Disease
Volume97
Issue number10
DOIs
Publication statusPublished - 2013

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United Arab Emirates
Phoenix dactylifera
Oman
mangoes
genetic techniques and protocols
Citrus
population genetics
genetic variation
internal transcribed spacers
gummosis
Lasiodiplodia
pathogens
acids
Mangifera indica
fruit crops
dieback
amplified fragment length polymorphism
cluster analysis
necrosis
pathogenicity

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Al-Sadi, A. M., Al-Wehaibi, A. N., Al-Shariqi, R. M., Al-Hammadi, M. S., Al-Hosni, I. A., Al-Mahmooli, I. H., & Al-Ghaithi, A. G. (2013). Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE. Plant Disease, 97(10), 1363-1369. https://doi.org/10.1094/PDIS-03-13-0245-RE

Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE. / Al-Sadi, A. M.; Al-Wehaibi, A. N.; Al-Shariqi, R. M.; Al-Hammadi, M. S.; Al-Hosni, I. A.; Al-Mahmooli, I. H.; Al-Ghaithi, A. G.

In: Plant Disease, Vol. 97, No. 10, 2013, p. 1363-1369.

Research output: Contribution to journalArticle

Al-Sadi, AM, Al-Wehaibi, AN, Al-Shariqi, RM, Al-Hammadi, MS, Al-Hosni, IA, Al-Mahmooli, IH & Al-Ghaithi, AG 2013, 'Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE', Plant Disease, vol. 97, no. 10, pp. 1363-1369. https://doi.org/10.1094/PDIS-03-13-0245-RE
Al-Sadi, A. M. ; Al-Wehaibi, A. N. ; Al-Shariqi, R. M. ; Al-Hammadi, M. S. ; Al-Hosni, I. A. ; Al-Mahmooli, I. H. ; Al-Ghaithi, A. G. / Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE. In: Plant Disease. 2013 ; Vol. 97, No. 10. pp. 1363-1369.
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abstract = "Lasiodiplodia is a common pathogen causing dieback, gummosis, or root necrosis on the three most important fruit crops in Oman and the United Arab Emirates (UAE): date palm (Phoenix dactylifera), Citrus spp., and mango (Mangifera indica). A study was conducted to examine diversity in 64 Lasiodiplodia isolates infecting date palm (24), Citrus (11), and mango (29) in Oman and the UAE. Identification based on sequences of the internal transcribed spacer (ITS) rDNA and EF1α gene showed that date palm isolates belonged to L. hormozganensis (75{\%} of isolates) and L. theobromae (25{\%}); Citrus isolates belonged to L. hormozganensis (45{\%}), L. theobromae (45{\%}), and L. iraniensis (10{\%}); and mango isolates belonged to L. theobromae (59{\%}), L. iraniensis (34{\%}), and L. hormozganensis (7{\%}). Amplified fragment length polymorphism (AFLP) fingerprinting of the 64 isolates using four primer pair combinations produced 64 genotypes and 972 polymorphic alleles. Cluster analysis separated the isolates into four clusters representing the three species. A higher level of genetic diversity was observed in L. iraniensis (0.3105) compared to L. hormozganensis (0.2503) and L. theobromae (0.2331) in Oman. Analysis of molecular variance (AMOVA) indicated the existence of low levels of genetic differentiation among date palm populations of L. hormozganensis obtained from Oman and the UAE (FST = 0.025) and among populations of L. hormozganensis (0.0485) and L. theobromae (0.0703) from date palm, Citrus, and mango. These findings imply a high rate of movement of L. hormozganensis and L. theobromae isolates among date palm, Citrus, and mango and between the two countries. Findings from the pathogenicity test supported the AMOVA analysis and suggested a lack of host specialization in L. hormozganensis, L. iraniensis, and L. theobromae on date palm, acid lime, and mango. Although this is the first record of L. hormozganensis and L. iraniensis in Oman, the relatively moderate level of genetic diversity in the two species compared to L. theobromae suggests that the two species have been in Oman for a long time but misidentified by morphology and ITS rDNA sequences as L. theobromae. This study is also the first record of date palm and acid lime as natural hosts for L. hormozganensis and the first record of L. hormozganensis in the UAE. The diversity in Lasiodiplodia species affecting date palm, Citrus, and mango in Oman and the UAE should be taken into consideration when planning future management programs for diseases caused by these pathogens.",
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T1 - Population genetic analysis reveals diversity in Lasiodiplodia species infecting date palm, citrus, and mango in Oman and the UAE

AU - Al-Sadi, A. M.

AU - Al-Wehaibi, A. N.

AU - Al-Shariqi, R. M.

AU - Al-Hammadi, M. S.

AU - Al-Hosni, I. A.

AU - Al-Mahmooli, I. H.

AU - Al-Ghaithi, A. G.

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N2 - Lasiodiplodia is a common pathogen causing dieback, gummosis, or root necrosis on the three most important fruit crops in Oman and the United Arab Emirates (UAE): date palm (Phoenix dactylifera), Citrus spp., and mango (Mangifera indica). A study was conducted to examine diversity in 64 Lasiodiplodia isolates infecting date palm (24), Citrus (11), and mango (29) in Oman and the UAE. Identification based on sequences of the internal transcribed spacer (ITS) rDNA and EF1α gene showed that date palm isolates belonged to L. hormozganensis (75% of isolates) and L. theobromae (25%); Citrus isolates belonged to L. hormozganensis (45%), L. theobromae (45%), and L. iraniensis (10%); and mango isolates belonged to L. theobromae (59%), L. iraniensis (34%), and L. hormozganensis (7%). Amplified fragment length polymorphism (AFLP) fingerprinting of the 64 isolates using four primer pair combinations produced 64 genotypes and 972 polymorphic alleles. Cluster analysis separated the isolates into four clusters representing the three species. A higher level of genetic diversity was observed in L. iraniensis (0.3105) compared to L. hormozganensis (0.2503) and L. theobromae (0.2331) in Oman. Analysis of molecular variance (AMOVA) indicated the existence of low levels of genetic differentiation among date palm populations of L. hormozganensis obtained from Oman and the UAE (FST = 0.025) and among populations of L. hormozganensis (0.0485) and L. theobromae (0.0703) from date palm, Citrus, and mango. These findings imply a high rate of movement of L. hormozganensis and L. theobromae isolates among date palm, Citrus, and mango and between the two countries. Findings from the pathogenicity test supported the AMOVA analysis and suggested a lack of host specialization in L. hormozganensis, L. iraniensis, and L. theobromae on date palm, acid lime, and mango. Although this is the first record of L. hormozganensis and L. iraniensis in Oman, the relatively moderate level of genetic diversity in the two species compared to L. theobromae suggests that the two species have been in Oman for a long time but misidentified by morphology and ITS rDNA sequences as L. theobromae. This study is also the first record of date palm and acid lime as natural hosts for L. hormozganensis and the first record of L. hormozganensis in the UAE. The diversity in Lasiodiplodia species affecting date palm, Citrus, and mango in Oman and the UAE should be taken into consideration when planning future management programs for diseases caused by these pathogens.

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