Polyphasic characterization of cyanobacteria isolated from desert crusts, and the description of Desertifilum tharense gen. et sp. nov. (Oscillatoriales)

Pawan K. Dadheech, Raeid M M Abed, Huda Mahmoud, M. Krishna Mohan, Lothar Krienitz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Four new cyanobacterial strains isolated from biological desert crusts in Thar Desert, India were characterized using a polyphasic approach. The strains were designated to two mophotypes, but all strains exhibited identical 16S ribosomal RNA (rRNA) gene sequences. On the basis of 16S rRNA phylogenetic reconstruction, the strains belonged to the Oscillatoriales order and formed a coherent cluster in the phylogenetic tree, with more than 5% sequence divergence to the closest relative belonging to a species of Microcoleus Desmazières ex Gomont. Our strains were different from the genus Microcoleus in phenotypic characters such as organization of thallus, trichome width, cell shape, gas vesicle, thylakoid arrangement and habitat. Although the strains shared some morphological similarities with members of Phormidiaceae, they differed in thylakoid pattern and 16S rRNA gene sequences. The type strain (PD2001/TDC17T) grew up to 45°C with optimum growth between 30 and 35°C. The sharp decrease in growth of that strain at 1% salinity indicates its sensitivity to salts. Phylogenetic, morphological, ultrastuctural and physiological analyses demonstrated that the investigated strains represented a novel cyanobacterial genus, for which the name Desertifilum tharense gen. et sp. nov. is proposed, with PD2001/TDC17T (5 CCAP 1420/4T 5 BISR/CYANO/61T) as the type strain.

Original languageEnglish
Pages (from-to)260-270
Number of pages11
JournalPhycologia
Volume51
Issue number3
DOIs
Publication statusPublished - May 2012

Fingerprint

Oscillatoriales
Cyanobacteria
cyanobacterium
deserts
desert
crust
phylogenetics
RNA
new species
trichome
gene
vesicle
Microcoleus
divergence
salt
salinity
thylakoids
habitat
phylogeny
gas

Keywords

  • 16S rRNA gene
  • Cyanobacteria
  • Desert crust
  • Desertifilum tharense gen. et sp. nov.
  • Oscillatoriales
  • Polyphasic

ASJC Scopus subject areas

  • Aquatic Science
  • Plant Science

Cite this

Polyphasic characterization of cyanobacteria isolated from desert crusts, and the description of Desertifilum tharense gen. et sp. nov. (Oscillatoriales). / Dadheech, Pawan K.; Abed, Raeid M M; Mahmoud, Huda; Krishna Mohan, M.; Krienitz, Lothar.

In: Phycologia, Vol. 51, No. 3, 05.2012, p. 260-270.

Research output: Contribution to journalArticle

@article{baf8816705334819ade9f1ec3a57be62,
title = "Polyphasic characterization of cyanobacteria isolated from desert crusts, and the description of Desertifilum tharense gen. et sp. nov. (Oscillatoriales)",
abstract = "Four new cyanobacterial strains isolated from biological desert crusts in Thar Desert, India were characterized using a polyphasic approach. The strains were designated to two mophotypes, but all strains exhibited identical 16S ribosomal RNA (rRNA) gene sequences. On the basis of 16S rRNA phylogenetic reconstruction, the strains belonged to the Oscillatoriales order and formed a coherent cluster in the phylogenetic tree, with more than 5{\%} sequence divergence to the closest relative belonging to a species of Microcoleus Desmazi{\`e}res ex Gomont. Our strains were different from the genus Microcoleus in phenotypic characters such as organization of thallus, trichome width, cell shape, gas vesicle, thylakoid arrangement and habitat. Although the strains shared some morphological similarities with members of Phormidiaceae, they differed in thylakoid pattern and 16S rRNA gene sequences. The type strain (PD2001/TDC17T) grew up to 45°C with optimum growth between 30 and 35°C. The sharp decrease in growth of that strain at 1{\%} salinity indicates its sensitivity to salts. Phylogenetic, morphological, ultrastuctural and physiological analyses demonstrated that the investigated strains represented a novel cyanobacterial genus, for which the name Desertifilum tharense gen. et sp. nov. is proposed, with PD2001/TDC17T (5 CCAP 1420/4T 5 BISR/CYANO/61T) as the type strain.",
keywords = "16S rRNA gene, Cyanobacteria, Desert crust, Desertifilum tharense gen. et sp. nov., Oscillatoriales, Polyphasic",
author = "Dadheech, {Pawan K.} and Abed, {Raeid M M} and Huda Mahmoud and {Krishna Mohan}, M. and Lothar Krienitz",
year = "2012",
month = "5",
doi = "10.2216/09-51.1",
language = "English",
volume = "51",
pages = "260--270",
journal = "Phycologia",
issn = "0031-8884",
publisher = "International Phycological Society",
number = "3",

}

TY - JOUR

T1 - Polyphasic characterization of cyanobacteria isolated from desert crusts, and the description of Desertifilum tharense gen. et sp. nov. (Oscillatoriales)

AU - Dadheech, Pawan K.

AU - Abed, Raeid M M

AU - Mahmoud, Huda

AU - Krishna Mohan, M.

AU - Krienitz, Lothar

PY - 2012/5

Y1 - 2012/5

N2 - Four new cyanobacterial strains isolated from biological desert crusts in Thar Desert, India were characterized using a polyphasic approach. The strains were designated to two mophotypes, but all strains exhibited identical 16S ribosomal RNA (rRNA) gene sequences. On the basis of 16S rRNA phylogenetic reconstruction, the strains belonged to the Oscillatoriales order and formed a coherent cluster in the phylogenetic tree, with more than 5% sequence divergence to the closest relative belonging to a species of Microcoleus Desmazières ex Gomont. Our strains were different from the genus Microcoleus in phenotypic characters such as organization of thallus, trichome width, cell shape, gas vesicle, thylakoid arrangement and habitat. Although the strains shared some morphological similarities with members of Phormidiaceae, they differed in thylakoid pattern and 16S rRNA gene sequences. The type strain (PD2001/TDC17T) grew up to 45°C with optimum growth between 30 and 35°C. The sharp decrease in growth of that strain at 1% salinity indicates its sensitivity to salts. Phylogenetic, morphological, ultrastuctural and physiological analyses demonstrated that the investigated strains represented a novel cyanobacterial genus, for which the name Desertifilum tharense gen. et sp. nov. is proposed, with PD2001/TDC17T (5 CCAP 1420/4T 5 BISR/CYANO/61T) as the type strain.

AB - Four new cyanobacterial strains isolated from biological desert crusts in Thar Desert, India were characterized using a polyphasic approach. The strains were designated to two mophotypes, but all strains exhibited identical 16S ribosomal RNA (rRNA) gene sequences. On the basis of 16S rRNA phylogenetic reconstruction, the strains belonged to the Oscillatoriales order and formed a coherent cluster in the phylogenetic tree, with more than 5% sequence divergence to the closest relative belonging to a species of Microcoleus Desmazières ex Gomont. Our strains were different from the genus Microcoleus in phenotypic characters such as organization of thallus, trichome width, cell shape, gas vesicle, thylakoid arrangement and habitat. Although the strains shared some morphological similarities with members of Phormidiaceae, they differed in thylakoid pattern and 16S rRNA gene sequences. The type strain (PD2001/TDC17T) grew up to 45°C with optimum growth between 30 and 35°C. The sharp decrease in growth of that strain at 1% salinity indicates its sensitivity to salts. Phylogenetic, morphological, ultrastuctural and physiological analyses demonstrated that the investigated strains represented a novel cyanobacterial genus, for which the name Desertifilum tharense gen. et sp. nov. is proposed, with PD2001/TDC17T (5 CCAP 1420/4T 5 BISR/CYANO/61T) as the type strain.

KW - 16S rRNA gene

KW - Cyanobacteria

KW - Desert crust

KW - Desertifilum tharense gen. et sp. nov.

KW - Oscillatoriales

KW - Polyphasic

UR - http://www.scopus.com/inward/record.url?scp=84861022023&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84861022023&partnerID=8YFLogxK

U2 - 10.2216/09-51.1

DO - 10.2216/09-51.1

M3 - Article

AN - SCOPUS:84861022023

VL - 51

SP - 260

EP - 270

JO - Phycologia

JF - Phycologia

SN - 0031-8884

IS - 3

ER -