Polyphasic characterization of benthic, moderately halophilic, moderately thermophilic cyanobacteria with very thin trichomes and the proposal of Halomicronema excentricum gen. nov., sp. nov.

Raeid M M Abed, Ferran Garcia-Pichel, Mariona Hernández-Mariné

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

A new genus of moderately halophilic, moderately halotolerant and moderately thermophilic cyanobacteria with very thin trichomes is described. The four strains included in this genus were isolated from benthic microbial mats in a man-made hypersaline pond. Trichomes were around 1 μm thick, with small constrictions at the cross-walls and diffluent colorless sheaths. Thylakoids were parallel to the cell wall, but thylakoids and nucleoid were often excentrically arranged within the cytoplasm with respect to the main trichome axis. Strains grew at between 3.2 and 12-15% (w/v) salinity with optima between 3.2 and 12%. They showed lower temperature limits around 20°C and upper limits between 45 and 50°C, with optima between 28 and 45-50°C. Carotenoid and mycosporine amino-acid complements were identical among strains. Phylogenetic analyses based on 16S rRNA gene sequence showed that all strains were closely related (99% or higher similarity) and distantly related to other cyanobacteria (91% or lower similarity). We propose the new genus and species Halomicronema excentricum for these strains. The type strain is TFEP1.

Original languageEnglish
Pages (from-to)361-370
Number of pages10
JournalArchives of Microbiology
Volume177
Issue number5
DOIs
Publication statusPublished - 2002

Fingerprint

Trichomes
Cyanobacteria
Thylakoids
Salinity
Carotenoids
rRNA Genes
Constriction
Cell Wall
Cytoplasm
Amino Acids
Ponds
Temperature
Genes
Cells

Keywords

  • 16S rRNA
  • Carotenoids
  • Halomicronema
  • Halotolerance
  • Hypersaline environments
  • Microbial mats
  • Phormidium, Leptolyngbya
  • Phylogeny
  • Polyphasic taxonomy
  • Thermotolerance

ASJC Scopus subject areas

  • Microbiology

Cite this

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title = "Polyphasic characterization of benthic, moderately halophilic, moderately thermophilic cyanobacteria with very thin trichomes and the proposal of Halomicronema excentricum gen. nov., sp. nov.",
abstract = "A new genus of moderately halophilic, moderately halotolerant and moderately thermophilic cyanobacteria with very thin trichomes is described. The four strains included in this genus were isolated from benthic microbial mats in a man-made hypersaline pond. Trichomes were around 1 μm thick, with small constrictions at the cross-walls and diffluent colorless sheaths. Thylakoids were parallel to the cell wall, but thylakoids and nucleoid were often excentrically arranged within the cytoplasm with respect to the main trichome axis. Strains grew at between 3.2 and 12-15{\%} (w/v) salinity with optima between 3.2 and 12{\%}. They showed lower temperature limits around 20°C and upper limits between 45 and 50°C, with optima between 28 and 45-50°C. Carotenoid and mycosporine amino-acid complements were identical among strains. Phylogenetic analyses based on 16S rRNA gene sequence showed that all strains were closely related (99{\%} or higher similarity) and distantly related to other cyanobacteria (91{\%} or lower similarity). We propose the new genus and species Halomicronema excentricum for these strains. The type strain is TFEP1.",
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AU - Hernández-Mariné, Mariona

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N2 - A new genus of moderately halophilic, moderately halotolerant and moderately thermophilic cyanobacteria with very thin trichomes is described. The four strains included in this genus were isolated from benthic microbial mats in a man-made hypersaline pond. Trichomes were around 1 μm thick, with small constrictions at the cross-walls and diffluent colorless sheaths. Thylakoids were parallel to the cell wall, but thylakoids and nucleoid were often excentrically arranged within the cytoplasm with respect to the main trichome axis. Strains grew at between 3.2 and 12-15% (w/v) salinity with optima between 3.2 and 12%. They showed lower temperature limits around 20°C and upper limits between 45 and 50°C, with optima between 28 and 45-50°C. Carotenoid and mycosporine amino-acid complements were identical among strains. Phylogenetic analyses based on 16S rRNA gene sequence showed that all strains were closely related (99% or higher similarity) and distantly related to other cyanobacteria (91% or lower similarity). We propose the new genus and species Halomicronema excentricum for these strains. The type strain is TFEP1.

AB - A new genus of moderately halophilic, moderately halotolerant and moderately thermophilic cyanobacteria with very thin trichomes is described. The four strains included in this genus were isolated from benthic microbial mats in a man-made hypersaline pond. Trichomes were around 1 μm thick, with small constrictions at the cross-walls and diffluent colorless sheaths. Thylakoids were parallel to the cell wall, but thylakoids and nucleoid were often excentrically arranged within the cytoplasm with respect to the main trichome axis. Strains grew at between 3.2 and 12-15% (w/v) salinity with optima between 3.2 and 12%. They showed lower temperature limits around 20°C and upper limits between 45 and 50°C, with optima between 28 and 45-50°C. Carotenoid and mycosporine amino-acid complements were identical among strains. Phylogenetic analyses based on 16S rRNA gene sequence showed that all strains were closely related (99% or higher similarity) and distantly related to other cyanobacteria (91% or lower similarity). We propose the new genus and species Halomicronema excentricum for these strains. The type strain is TFEP1.

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