Polyhydroxyalkanoate biosynthesis and simplified polymer recovery by a novel moderately halophilic bacterium isolated from hypersaline microbial mats

D. N. Rathi, H. G. Amir, R. M M Abed, A. Kosugi, T. Arai, O. Sulaiman, R. Hashim, K. Sudesh

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Aims: Halophilic micro-organisms have received much interest because of their potential biotechnological applications, among which is the capability of some strains to synthesize polyhydroxyalkanoates (PHA). Halomonas sp. SK5, which was isolated from hypersaline microbial mats, accumulated intracellular granules of poly(3-hydroxybutyrate) [P(3HB)] in modified accumulation medium supplemented with 10% (w/v) salinity and 3% (w/v) glucose. Methods and Results: A cell density of approximately 3·0 g l-1 was attained in this culture which yielded 48 wt% P(3HB). The bacterial strain was also capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] when cofed with relevant precursors. Feeding with sodium valerate (0·7 mol l-1 carbon) at various time intervals within 36 h resulted in 3HV molar fractions ranging from 6 up to 54 mol%. Oil palm trunk sap (OPTS) and seawater as the carbon source and culture medium respectively facilitated a significant accumulation of P(3HB). Simplified downstream processing based on osmotic lysis in the presence of alkali/detergent for both dry and wet biomass resulted in approximately 90-100% recovery of polymers with purity as high as 90%. Weight-average molecular weight (Mw) of the polymers recovered was in the range of 1-2 × 106. Conclusions: Halomonas sp. SK5 was able to synthesize P(3HB) homopolymer as well as P(3HB-co-3HV) copolymer from various carbon sources. Significance and Impact of the Study: This is the first time a comprehensive study of both production and downstream processing is reported for Halomonas spp.

Original languageEnglish
Pages (from-to)384-395
Number of pages12
JournalJournal of Applied Microbiology
Volume114
Issue number2
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Polyhydroxyalkanoates
Halomonas
Polymers
Bacteria
Carbon
Valerates
3-Hydroxybutyric Acid
Salinity
Seawater
Alkalies
Detergents
Biomass
Culture Media
Oils
Cell Count
Molecular Weight
Sodium
Weights and Measures
Glucose
poly-beta-hydroxybutyrate

Keywords

  • Halomonas sp
  • Halophilic micro-organisms
  • Oil palm trunk sap
  • Osmotic lysis
  • Polyhydroxyalkanoate

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Biotechnology

Cite this

Polyhydroxyalkanoate biosynthesis and simplified polymer recovery by a novel moderately halophilic bacterium isolated from hypersaline microbial mats. / Rathi, D. N.; Amir, H. G.; Abed, R. M M; Kosugi, A.; Arai, T.; Sulaiman, O.; Hashim, R.; Sudesh, K.

In: Journal of Applied Microbiology, Vol. 114, No. 2, 02.2013, p. 384-395.

Research output: Contribution to journalArticle

Rathi, D. N. ; Amir, H. G. ; Abed, R. M M ; Kosugi, A. ; Arai, T. ; Sulaiman, O. ; Hashim, R. ; Sudesh, K. / Polyhydroxyalkanoate biosynthesis and simplified polymer recovery by a novel moderately halophilic bacterium isolated from hypersaline microbial mats. In: Journal of Applied Microbiology. 2013 ; Vol. 114, No. 2. pp. 384-395.
@article{e012b0fe4e6e405cbf7dc4646d6e3dad,
title = "Polyhydroxyalkanoate biosynthesis and simplified polymer recovery by a novel moderately halophilic bacterium isolated from hypersaline microbial mats",
abstract = "Aims: Halophilic micro-organisms have received much interest because of their potential biotechnological applications, among which is the capability of some strains to synthesize polyhydroxyalkanoates (PHA). Halomonas sp. SK5, which was isolated from hypersaline microbial mats, accumulated intracellular granules of poly(3-hydroxybutyrate) [P(3HB)] in modified accumulation medium supplemented with 10{\%} (w/v) salinity and 3{\%} (w/v) glucose. Methods and Results: A cell density of approximately 3·0 g l-1 was attained in this culture which yielded 48 wt{\%} P(3HB). The bacterial strain was also capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] when cofed with relevant precursors. Feeding with sodium valerate (0·7 mol l-1 carbon) at various time intervals within 36 h resulted in 3HV molar fractions ranging from 6 up to 54 mol{\%}. Oil palm trunk sap (OPTS) and seawater as the carbon source and culture medium respectively facilitated a significant accumulation of P(3HB). Simplified downstream processing based on osmotic lysis in the presence of alkali/detergent for both dry and wet biomass resulted in approximately 90-100{\%} recovery of polymers with purity as high as 90{\%}. Weight-average molecular weight (Mw) of the polymers recovered was in the range of 1-2 × 106. Conclusions: Halomonas sp. SK5 was able to synthesize P(3HB) homopolymer as well as P(3HB-co-3HV) copolymer from various carbon sources. Significance and Impact of the Study: This is the first time a comprehensive study of both production and downstream processing is reported for Halomonas spp.",
keywords = "Halomonas sp, Halophilic micro-organisms, Oil palm trunk sap, Osmotic lysis, Polyhydroxyalkanoate",
author = "Rathi, {D. N.} and Amir, {H. G.} and Abed, {R. M M} and A. Kosugi and T. Arai and O. Sulaiman and R. Hashim and K. Sudesh",
year = "2013",
month = "2",
doi = "10.1111/jam.12083",
language = "English",
volume = "114",
pages = "384--395",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - Polyhydroxyalkanoate biosynthesis and simplified polymer recovery by a novel moderately halophilic bacterium isolated from hypersaline microbial mats

AU - Rathi, D. N.

AU - Amir, H. G.

AU - Abed, R. M M

AU - Kosugi, A.

AU - Arai, T.

AU - Sulaiman, O.

AU - Hashim, R.

AU - Sudesh, K.

PY - 2013/2

Y1 - 2013/2

N2 - Aims: Halophilic micro-organisms have received much interest because of their potential biotechnological applications, among which is the capability of some strains to synthesize polyhydroxyalkanoates (PHA). Halomonas sp. SK5, which was isolated from hypersaline microbial mats, accumulated intracellular granules of poly(3-hydroxybutyrate) [P(3HB)] in modified accumulation medium supplemented with 10% (w/v) salinity and 3% (w/v) glucose. Methods and Results: A cell density of approximately 3·0 g l-1 was attained in this culture which yielded 48 wt% P(3HB). The bacterial strain was also capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] when cofed with relevant precursors. Feeding with sodium valerate (0·7 mol l-1 carbon) at various time intervals within 36 h resulted in 3HV molar fractions ranging from 6 up to 54 mol%. Oil palm trunk sap (OPTS) and seawater as the carbon source and culture medium respectively facilitated a significant accumulation of P(3HB). Simplified downstream processing based on osmotic lysis in the presence of alkali/detergent for both dry and wet biomass resulted in approximately 90-100% recovery of polymers with purity as high as 90%. Weight-average molecular weight (Mw) of the polymers recovered was in the range of 1-2 × 106. Conclusions: Halomonas sp. SK5 was able to synthesize P(3HB) homopolymer as well as P(3HB-co-3HV) copolymer from various carbon sources. Significance and Impact of the Study: This is the first time a comprehensive study of both production and downstream processing is reported for Halomonas spp.

AB - Aims: Halophilic micro-organisms have received much interest because of their potential biotechnological applications, among which is the capability of some strains to synthesize polyhydroxyalkanoates (PHA). Halomonas sp. SK5, which was isolated from hypersaline microbial mats, accumulated intracellular granules of poly(3-hydroxybutyrate) [P(3HB)] in modified accumulation medium supplemented with 10% (w/v) salinity and 3% (w/v) glucose. Methods and Results: A cell density of approximately 3·0 g l-1 was attained in this culture which yielded 48 wt% P(3HB). The bacterial strain was also capable of synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] when cofed with relevant precursors. Feeding with sodium valerate (0·7 mol l-1 carbon) at various time intervals within 36 h resulted in 3HV molar fractions ranging from 6 up to 54 mol%. Oil palm trunk sap (OPTS) and seawater as the carbon source and culture medium respectively facilitated a significant accumulation of P(3HB). Simplified downstream processing based on osmotic lysis in the presence of alkali/detergent for both dry and wet biomass resulted in approximately 90-100% recovery of polymers with purity as high as 90%. Weight-average molecular weight (Mw) of the polymers recovered was in the range of 1-2 × 106. Conclusions: Halomonas sp. SK5 was able to synthesize P(3HB) homopolymer as well as P(3HB-co-3HV) copolymer from various carbon sources. Significance and Impact of the Study: This is the first time a comprehensive study of both production and downstream processing is reported for Halomonas spp.

KW - Halomonas sp

KW - Halophilic micro-organisms

KW - Oil palm trunk sap

KW - Osmotic lysis

KW - Polyhydroxyalkanoate

UR - http://www.scopus.com/inward/record.url?scp=84872409455&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84872409455&partnerID=8YFLogxK

U2 - 10.1111/jam.12083

DO - 10.1111/jam.12083

M3 - Article

VL - 114

SP - 384

EP - 395

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 2

ER -