TY - JOUR
T1 - Platelet activation in pregnancy-induced hypertension
AU - Karalis, Ioannis
AU - Nadar, Sunil K.
AU - Al Yemeni, Eman
AU - Blann, Andrew D.
AU - Lip, Gregory Y H
PY - 2005
Y1 - 2005
N2 - Background: Although excess platelet activation, as indicated by increased plasma beta thromboglobulin (β-TG), has been shown in pregnancy-induced hypertension (PIH), platelet adhesion, platelet morphology and a comparison of platelet and soluble (plasma) levels of the adhesion molecules P-selectin (pPsel and sPsel, respectively) have not been studied. Methods: We conducted a cross-sectional study of 35 consecutive women with PIH (age 31±6 years), 31 consecutive women with normotensive pregnancies (age 29±5 years) and 30 normotensive non pregnant women (age 30±5 years). Platelet adhesion was studied in vitro by binding to fibrinogen-coated microwells, platelet morphology [mass and volume by flow cytometry], whole-platelet P-selectin (pPsel) by ELISA of the lysate of 2×108 cells, and the plasma markers soluble P-selectin (sP-sel) and β-TG, by ELISA. Results: The women with PIH had significantly raised sPsel, pPsel and (as expected) β-TG (all p<0.05), when compared to the normotensive pregnant women and controls. However, in PIH platelet adhesion was similar to that in the normotensive pregnancy, but still higher than the normal controls (p<0.001). There was no difference among the three groups with respect to platelet mass and volume. pPsel and platelet adhesion correlated with gestational age and with systolic and diastolic blood pressure (all p<0.05). Conclusions: Increased platelet activation and adhesion develop during normal pregnancy, with some indices being further altered in PIH.
AB - Background: Although excess platelet activation, as indicated by increased plasma beta thromboglobulin (β-TG), has been shown in pregnancy-induced hypertension (PIH), platelet adhesion, platelet morphology and a comparison of platelet and soluble (plasma) levels of the adhesion molecules P-selectin (pPsel and sPsel, respectively) have not been studied. Methods: We conducted a cross-sectional study of 35 consecutive women with PIH (age 31±6 years), 31 consecutive women with normotensive pregnancies (age 29±5 years) and 30 normotensive non pregnant women (age 30±5 years). Platelet adhesion was studied in vitro by binding to fibrinogen-coated microwells, platelet morphology [mass and volume by flow cytometry], whole-platelet P-selectin (pPsel) by ELISA of the lysate of 2×108 cells, and the plasma markers soluble P-selectin (sP-sel) and β-TG, by ELISA. Results: The women with PIH had significantly raised sPsel, pPsel and (as expected) β-TG (all p<0.05), when compared to the normotensive pregnant women and controls. However, in PIH platelet adhesion was similar to that in the normotensive pregnancy, but still higher than the normal controls (p<0.001). There was no difference among the three groups with respect to platelet mass and volume. pPsel and platelet adhesion correlated with gestational age and with systolic and diastolic blood pressure (all p<0.05). Conclusions: Increased platelet activation and adhesion develop during normal pregnancy, with some indices being further altered in PIH.
KW - P-selectin
KW - Platelet activation
KW - Platelet adhesion
KW - Preeclampsia
KW - Pregnancy-induced hypertension
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U2 - 10.1016/j.thromres.2005.01.009
DO - 10.1016/j.thromres.2005.01.009
M3 - Article
C2 - 16122550
AN - SCOPUS:23944475134
VL - 116
SP - 377
EP - 383
JO - Thrombosis Research
JF - Thrombosis Research
SN - 0049-3848
IS - 5
ER -