Platelet activation in pregnancy-induced hypertension

Ioannis Karalis, Sunil K. Nadar, Eman Al Yemeni, Andrew D. Blann, Gregory Y H Lip

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Background: Although excess platelet activation, as indicated by increased plasma beta thromboglobulin (β-TG), has been shown in pregnancy-induced hypertension (PIH), platelet adhesion, platelet morphology and a comparison of platelet and soluble (plasma) levels of the adhesion molecules P-selectin (pPsel and sPsel, respectively) have not been studied. Methods: We conducted a cross-sectional study of 35 consecutive women with PIH (age 31±6 years), 31 consecutive women with normotensive pregnancies (age 29±5 years) and 30 normotensive non pregnant women (age 30±5 years). Platelet adhesion was studied in vitro by binding to fibrinogen-coated microwells, platelet morphology [mass and volume by flow cytometry], whole-platelet P-selectin (pPsel) by ELISA of the lysate of 2×108 cells, and the plasma markers soluble P-selectin (sP-sel) and β-TG, by ELISA. Results: The women with PIH had significantly raised sPsel, pPsel and (as expected) β-TG (all p<0.05), when compared to the normotensive pregnant women and controls. However, in PIH platelet adhesion was similar to that in the normotensive pregnancy, but still higher than the normal controls (p<0.001). There was no difference among the three groups with respect to platelet mass and volume. pPsel and platelet adhesion correlated with gestational age and with systolic and diastolic blood pressure (all p<0.05). Conclusions: Increased platelet activation and adhesion develop during normal pregnancy, with some indices being further altered in PIH.

Original languageEnglish
Pages (from-to)377-383
Number of pages7
JournalThrombosis Research
Volume116
Issue number5
DOIs
Publication statusPublished - 2005

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Pregnancy Induced Hypertension
Platelet Activation
Blood Platelets
P-Selectin
Pregnancy
Pregnant Women
Enzyme-Linked Immunosorbent Assay
beta-Thromboglobulin
Blood Pressure
Plasma Cells
Fibrinogen
Gestational Age
Flow Cytometry
Cross-Sectional Studies

Keywords

  • P-selectin
  • Platelet activation
  • Platelet adhesion
  • Preeclampsia
  • Pregnancy-induced hypertension

ASJC Scopus subject areas

  • Hematology

Cite this

Platelet activation in pregnancy-induced hypertension. / Karalis, Ioannis; Nadar, Sunil K.; Al Yemeni, Eman; Blann, Andrew D.; Lip, Gregory Y H.

In: Thrombosis Research, Vol. 116, No. 5, 2005, p. 377-383.

Research output: Contribution to journalArticle

Karalis, I, Nadar, SK, Al Yemeni, E, Blann, AD & Lip, GYH 2005, 'Platelet activation in pregnancy-induced hypertension', Thrombosis Research, vol. 116, no. 5, pp. 377-383. https://doi.org/10.1016/j.thromres.2005.01.009
Karalis, Ioannis ; Nadar, Sunil K. ; Al Yemeni, Eman ; Blann, Andrew D. ; Lip, Gregory Y H. / Platelet activation in pregnancy-induced hypertension. In: Thrombosis Research. 2005 ; Vol. 116, No. 5. pp. 377-383.
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