Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy

Amani M A Bakhiet, Abdel Muhsin A Abdel-Muhsin, Salah Eldin G Elzaki, Zainab Al-Hashami, Hamida S. Albarwani, Badar A. AlQamashoui, Salama Al-Hamidhi, Mohamed A. Idris, Atif A. Elagib, Albano Beja-Pereira, Hamza A. Babiker

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Over the past decade, Sudan has stepped up malaria control backed by WHO, and this has resulted in significant reduction in parasite rate, malaria morbidity and mortality. The present study analyzed Plasmodium falciparum parasites in four geographical separated areas, to examine whether the success in malaria control following the use of artemisinin-based combination therapy (ACT) has disrupted the population structure and evolution of the parasite.We examined 319 P. falciparum isolates collected between October 2009 and October 2012 in four different areas in Sudan (Jazira [central Sudan], Southern Darfur [western Sudan], Upper Nile [southern Sudan] and Kasala [eastern Sudan]). Twelve microsatellites were analyzed for allelic diversity, multi-locus haplotype and inter-population differentiation. Level of diversity was compared to that detected for three of the above microsatellites among P. falciparum parasites in central and eastern Sudan in 1999, prior to introduction of ACT.Diversity at each locus (unbiased heterozygosity [H]) was high in all areas (Jazira, H=0.67), (Southern Darfur, H=0.71), (Upper Nile, H=0.71), and (Kasala, H=0.63). Microsatellites were distributed widely and private alleles, detected in a single population, were rare. The extent of diversity in the above sites was similar to that seen, in 1999, in central (Khartoum, H=0.73) and eastern Sudan (Gedaref, H=0.75). Significant Linkage disequilibrium (LD) was observed between the microsatellites in all populations. Pairwise FST analysis revealed that parasites in the four areas could be considered as one population. However, the parasites in Sudan clustered away from parasites in West Africa and the Arabian Peninsula.Despite marked reduction in malaria risk in Sudan, the extent of diversity and parasite genetic structure are indicative of a large population size. Further considerable reduction in transmission would be needed before fragmented sub-population can be seen. In addition, the large divergence of P. falciparum in Sudan from West Africa and Arabian Peninsula populations may result from differential evolutionary pressures acting at the population level, which shall be considered in eradication plans.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalActa Tropica
Volume148
DOIs
Publication statusPublished - Aug 1 2015

Fingerprint

artemisinin
Sudan
Plasmodium falciparum
population structure
therapeutics
Parasites
parasites
Population
Microsatellite Repeats
malaria
Malaria
Therapeutics
microsatellite repeats
Western Africa
artemisinine
loci
Genetic Structures
Linkage Disequilibrium
linkage disequilibrium
Population Density

Keywords

  • Genetic diversity
  • Plasmodium falciparum
  • Population structure
  • Sudan

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

Bakhiet, A. M. A., Abdel-Muhsin, A. M. A., Elzaki, S. E. G., Al-Hashami, Z., Albarwani, H. S., AlQamashoui, B. A., ... Babiker, H. A. (2015). Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy. Acta Tropica, 148, 97-104. https://doi.org/10.1016/j.actatropica.2015.04.013

Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy. / Bakhiet, Amani M A; Abdel-Muhsin, Abdel Muhsin A; Elzaki, Salah Eldin G; Al-Hashami, Zainab; Albarwani, Hamida S.; AlQamashoui, Badar A.; Al-Hamidhi, Salama; Idris, Mohamed A.; Elagib, Atif A.; Beja-Pereira, Albano; Babiker, Hamza A.

In: Acta Tropica, Vol. 148, 01.08.2015, p. 97-104.

Research output: Contribution to journalArticle

Bakhiet, AMA, Abdel-Muhsin, AMA, Elzaki, SEG, Al-Hashami, Z, Albarwani, HS, AlQamashoui, BA, Al-Hamidhi, S, Idris, MA, Elagib, AA, Beja-Pereira, A & Babiker, HA 2015, 'Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy', Acta Tropica, vol. 148, pp. 97-104. https://doi.org/10.1016/j.actatropica.2015.04.013
Bakhiet AMA, Abdel-Muhsin AMA, Elzaki SEG, Al-Hashami Z, Albarwani HS, AlQamashoui BA et al. Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy. Acta Tropica. 2015 Aug 1;148:97-104. https://doi.org/10.1016/j.actatropica.2015.04.013
Bakhiet, Amani M A ; Abdel-Muhsin, Abdel Muhsin A ; Elzaki, Salah Eldin G ; Al-Hashami, Zainab ; Albarwani, Hamida S. ; AlQamashoui, Badar A. ; Al-Hamidhi, Salama ; Idris, Mohamed A. ; Elagib, Atif A. ; Beja-Pereira, Albano ; Babiker, Hamza A. / Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy. In: Acta Tropica. 2015 ; Vol. 148. pp. 97-104.
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