Phytochemical, antimicrobial and cytotoxic evaluation of Ziziphus spina-christi (L.) stem bark

Essam Nabih Ads, Saravanan Rajendrasozhan, Syed Imran Hassan, Sherif Mohamed Sayed Sharawy, Jamal Ragheb Humaidi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Ziziphus spina-christi (L.), commonly called jujube, was evaluated for their phytochemical content in the stem bark as well as the antimicrobial and cytotoxic activities. Z. spina-christi bark was extracted with ethanol and the extract was partitioned between aqueous layer and ethyl acetate layer. The ethyl acetate extract was defatted using diethyl ether and used for GC-MS analysis. The aqueous layer was further extracted with ethyl acetate and drop wise addition ammonia solution to obtain alkaline ethyl acetate extract. Both ethyl acetate extract and alkaline ethyl acetate extract were tested for phytochemical content by qualitative analysis and evaluated for their biological activities such as antimicrobial (by agar well diffusion assay and minimum inhibitory concentration determination) and cytotoxic activities (by MTT assay in cell culture). Phytochemical analysis indicates the presence of tannins, flavonoids, terpernoids, saponin glycosides and alkaloids in Z. spina-christi. Ethyl acetate and alkaline ethyl acetate extracts showed antifungal (against Aspergillus fumigatus, Syncephalastrum racemosum and Geotricum candidum), antibacterial (against Streptococcus pneumoniae, Bacillis subtilis and Escherichia coli), and cytotoxic effects (against colon and breast carcinoma cells). In comparison, alkaline ethyl acetate extract showed significantly (p<0.05) higher antimicrobial activity (against A. fumigatus, S. racemosum and S. pneumoniae) and cytotoxic activities as compared to ethyl acetate extract (IC50 of alkaline ethyl acetate and ethyl acetate extracts=196 and 400 μg/well against colon carcinoma cells and 164 and 397 μg/well against breast carcinoma cells, respectively).

Original languageEnglish
Pages (from-to)6646-6653
Number of pages8
JournalBiomedical Research (India)
Volume28
Issue number15
Publication statusPublished - 2017

Fingerprint

Ziziphus
Phytochemicals
Aspergillus fumigatus
Cells
Streptococcus pneumoniae
ethyl acetate
Assays
Colon
Breast Neoplasms
Aspergillus
Tannins
Saponins
Microbial Sensitivity Tests
Glycosides
Bioactivity
Cell culture
Ammonia
Alkaloids
Flavonoids
Ether

Keywords

  • Antibacterial
  • Antifungal
  • Antitumor
  • Cytotoxicity
  • Jujube
  • Stem bark

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Ads, E. N., Rajendrasozhan, S., Hassan, S. I., Sharawy, S. M. S., & Humaidi, J. R. (2017). Phytochemical, antimicrobial and cytotoxic evaluation of Ziziphus spina-christi (L.) stem bark. Biomedical Research (India), 28(15), 6646-6653.

Phytochemical, antimicrobial and cytotoxic evaluation of Ziziphus spina-christi (L.) stem bark. / Ads, Essam Nabih; Rajendrasozhan, Saravanan; Hassan, Syed Imran; Sharawy, Sherif Mohamed Sayed; Humaidi, Jamal Ragheb.

In: Biomedical Research (India), Vol. 28, No. 15, 2017, p. 6646-6653.

Research output: Contribution to journalArticle

Ads, EN, Rajendrasozhan, S, Hassan, SI, Sharawy, SMS & Humaidi, JR 2017, 'Phytochemical, antimicrobial and cytotoxic evaluation of Ziziphus spina-christi (L.) stem bark', Biomedical Research (India), vol. 28, no. 15, pp. 6646-6653.
Ads, Essam Nabih ; Rajendrasozhan, Saravanan ; Hassan, Syed Imran ; Sharawy, Sherif Mohamed Sayed ; Humaidi, Jamal Ragheb. / Phytochemical, antimicrobial and cytotoxic evaluation of Ziziphus spina-christi (L.) stem bark. In: Biomedical Research (India). 2017 ; Vol. 28, No. 15. pp. 6646-6653.
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