Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs

H. A. Agrama, M. R. Tuinstra

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Two DNA-based fingerprinting techniques, simple sequence repeats (SSR) and random amplified polymorphic DNA (RAPD) analyses, were applied in sorghum germplasm analysis to compare suitability for quantifying genetic diversity. Twenty-two sorghum genotypes, representing an array of germplasm sources with important agronomic traits, were assayed for polymorphism using 32 RAPD primers and 28 sets of sorghum SSR primers. The results indicated that SSR markers were highly polymorphic with an average of 4.5 alleles per primer. The RAPD primers were less polymorphic with nearly 40% of the fragments being monomorphic. An analysis of genetic diversity among sorghum lines indicated that the genetic distances calculated from SSR data were highly correlated with the distances based on the geographic origin and race classifications. Based on the results of these studies, SSR markers appear to be particularly useful for the estimation of genetic similarity among diverse genotypes of sorghum.

Original languageEnglish
Pages (from-to)339-351
Number of pages13
JournalAfrican Journal of Biotechnology
Volume2
Issue number10
Publication statusPublished - 2003

Fingerprint

Sorghum
Microsatellite Repeats
random amplified polymorphic DNA technique
microsatellite repeats
phylogeny
DNA Primers
DNA primers
germplasm
Genotype
genetic variation
DNA Fingerprinting
genotype
agronomic traits
provenance
genetic distance
Alleles
genetic polymorphism
alleles
taxonomy
DNA

Keywords

  • Cluster
  • Diversity
  • Polymorphism
  • RAPD
  • Sorghum
  • SSR

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Agronomy and Crop Science
  • Genetics

Cite this

Phylogenetic diversity and relationships among sorghum accessions using SSRs and RAPDs. / Agrama, H. A.; Tuinstra, M. R.

In: African Journal of Biotechnology, Vol. 2, No. 10, 2003, p. 339-351.

Research output: Contribution to journalArticle

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