TY - JOUR
T1 - Phylogenetic analysis of acute bee paralysis virus strains
AU - Bakonyi, Tamás
AU - Grabensteiner, Elvira
AU - Kolodziejek, Jolanta
AU - Rusvai, Miklós
AU - Topolska, Gražyna
AU - Ritter, Wolfgang
AU - Nowotny, Norbert
PY - 2002/12/1
Y1 - 2002/12/1
N2 - Reverse transcription-PCR assays have been established for a quick, sensitive, and specific diagnosis of acute bee paralysis virus (ABPV), a common virus of the honeybee (Apis mellifera), directly from clinical samples. A 3,071-nucleotide fragment of the ABPV genome, which includes the entire capsid polyprotein gene, was amplified from Austrian, German, Polish, and Hungarian ABPV samples and sequenced, and the sequences were compared. The alignment of a smaller fragment with ABPV sequences from the United States and the United Kingdom revealed nucleotide identity rates between 89 and 96%, respectively. Phylogenetic trees which display the molecular relationship between the viruses of different geographic origin were constructed.
AB - Reverse transcription-PCR assays have been established for a quick, sensitive, and specific diagnosis of acute bee paralysis virus (ABPV), a common virus of the honeybee (Apis mellifera), directly from clinical samples. A 3,071-nucleotide fragment of the ABPV genome, which includes the entire capsid polyprotein gene, was amplified from Austrian, German, Polish, and Hungarian ABPV samples and sequenced, and the sequences were compared. The alignment of a smaller fragment with ABPV sequences from the United States and the United Kingdom revealed nucleotide identity rates between 89 and 96%, respectively. Phylogenetic trees which display the molecular relationship between the viruses of different geographic origin were constructed.
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U2 - 10.1128/AEM.68.12.6446-6450.2002
DO - 10.1128/AEM.68.12.6446-6450.2002
M3 - Article
C2 - 12450876
AN - SCOPUS:0242500929
SN - 0099-2240
VL - 68
SP - 6446
EP - 6450
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 12
ER -