Phylogenetic analysis of acute bee paralysis virus strains

Tamás Bakonyi, Elvira Grabensteiner, Jolanta Kolodziejek, Miklós Rusvai, Gražyna Topolska, Wolfgang Ritter, Norbert Nowotny

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Reverse transcription-PCR assays have been established for a quick, sensitive, and specific diagnosis of acute bee paralysis virus (ABPV), a common virus of the honeybee (Apis mellifera), directly from clinical samples. A 3,071-nucleotide fragment of the ABPV genome, which includes the entire capsid polyprotein gene, was amplified from Austrian, German, Polish, and Hungarian ABPV samples and sequenced, and the sequences were compared. The alignment of a smaller fragment with ABPV sequences from the United States and the United Kingdom revealed nucleotide identity rates between 89 and 96%, respectively. Phylogenetic trees which display the molecular relationship between the viruses of different geographic origin were constructed.

Original languageEnglish
Pages (from-to)6446-6450
Number of pages5
JournalApplied and Environmental Microbiology
Volume68
Issue number12
DOIs
Publication statusPublished - Dec 1 2002

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

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  • Cite this

    Bakonyi, T., Grabensteiner, E., Kolodziejek, J., Rusvai, M., Topolska, G., Ritter, W., & Nowotny, N. (2002). Phylogenetic analysis of acute bee paralysis virus strains. Applied and Environmental Microbiology, 68(12), 6446-6450. https://doi.org/10.1128/AEM.68.12.6446-6450.2002