Pharmacological induction of larval settlement and metamorphosis in the blue mussel Mytilus edulis L.

Sergey V. Dobretsov, Pei Yuan Qian

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The blue mussel Mytilus edulis L. is an important aquaculture and fouling species in northern seas. Although the general role of chemical cues for settlement of larvae of the blue mussel has been proposed, few studies have focused on induction of settlement and metamorphosis by pharmacological agents. In this study, the induction of larval settlement of the blue mussel by pharmacological compounds was investigated through a series of laboratory experiments with an aim of identifying artificial cues for laboratory bioassay systems in fouling and antifouling research. Gamma-aminobutiric acid (GABA), dihydroxyphenyl L-alanine (DOPA), isobutyl methylxanthine (IBMX) and acetylcholine chloride (ACH) at 10-7-10-2 M as well as KCl at 10-40 mM K+ in excess of the level in normal seawater were tested for their inductive effect on larval settlement. In filtered seawater (FSW) + and GABA levels had no effect on larval settlement and metamorphosis. DOPA at 10-5 M and IBMX at 10-6-10-4 M induced 41-83% larval settlement and ACH at 10-7-10-5 M induced <40% larval settlement. While the highest settlement rates were observed after 48 h exposure to the chemicals, most of the larvae settled within 24 h. Compounds at concentrations of 10-3-10-2 M were either toxic to larvae or retarded the growth of the post-larvae shell. Juveniles resulting from induction by lower concentrations of chemicals had a very high survival rate, completed metamorphosis and grew as well as the juveniles that metamorphosed spontaneously. IBMX at 10-6-10-4 M and L-DOPA at 10-5 M are effective agents for induction of settlement and metamorphosis for future studies using juvenile M. edulis.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalBiofouling
Volume19
Issue number1
DOIs
Publication statusPublished - Feb 2003

Fingerprint

Mytilus edulis
larval settlement
metamorphosis
Pharmacology
Larva
Alanine
alanine
larva
larvae
Seawater
fouling
acetylcholine
Acetylcholine
Cues
chlorides
seawater
chloride
Aquaculture
chemical concentration
Acids

Keywords

  • Chemical cues
  • Growth
  • Larval settlement
  • Metamorphosis
  • Mortality
  • Mytilus edulis

ASJC Scopus subject areas

  • Aquatic Science
  • Biotechnology

Cite this

Pharmacological induction of larval settlement and metamorphosis in the blue mussel Mytilus edulis L. / Dobretsov, Sergey V.; Qian, Pei Yuan.

In: Biofouling, Vol. 19, No. 1, 02.2003, p. 57-63.

Research output: Contribution to journalArticle

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abstract = "The blue mussel Mytilus edulis L. is an important aquaculture and fouling species in northern seas. Although the general role of chemical cues for settlement of larvae of the blue mussel has been proposed, few studies have focused on induction of settlement and metamorphosis by pharmacological agents. In this study, the induction of larval settlement of the blue mussel by pharmacological compounds was investigated through a series of laboratory experiments with an aim of identifying artificial cues for laboratory bioassay systems in fouling and antifouling research. Gamma-aminobutiric acid (GABA), dihydroxyphenyl L-alanine (DOPA), isobutyl methylxanthine (IBMX) and acetylcholine chloride (ACH) at 10-7-10-2 M as well as KCl at 10-40 mM K+ in excess of the level in normal seawater were tested for their inductive effect on larval settlement. In filtered seawater (FSW) + and GABA levels had no effect on larval settlement and metamorphosis. DOPA at 10-5 M and IBMX at 10-6-10-4 M induced 41-83{\%} larval settlement and ACH at 10-7-10-5 M induced <40{\%} larval settlement. While the highest settlement rates were observed after 48 h exposure to the chemicals, most of the larvae settled within 24 h. Compounds at concentrations of 10-3-10-2 M were either toxic to larvae or retarded the growth of the post-larvae shell. Juveniles resulting from induction by lower concentrations of chemicals had a very high survival rate, completed metamorphosis and grew as well as the juveniles that metamorphosed spontaneously. IBMX at 10-6-10-4 M and L-DOPA at 10-5 M are effective agents for induction of settlement and metamorphosis for future studies using juvenile M. edulis.",
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