TY - JOUR
T1 - Pharmacological basis for the medicinal use of Psidium guajava leave in hyperactive gut disorders
AU - Shah, Abdul Jabbar
AU - Begum, Sabira
AU - Hassan, Syed Imran
AU - Ali, Syed Nawazish
AU - Siddiqui, Bina S.
AU - Gilani, Anwarul Hassan
PY - 2011
Y1 - 2011
N2 - Psidium guajava L. is reputed for its medicinal use in hyperactive gut disorders. This study was aimed to investigate mechanism responsible for its medicinal use in diarrhea and gut spasm. In castor oil-induced diarrheal model, the crude extract of P. guajava (100-1,000 mg/kg), provided 20.52-81.05% protection, similar to loperamide. In isolated rabbit jejunum preparations, crude extract was found more potent against high K+ than spontaneous precontractions, similar to verapamil, with EC50 values of 0.66 (0.44-0.97; n=8) and 2.28 mg/mL (1.43-3.62; n=8), respectively, suggests calcium channel blocking activity, as a possible mode of action. The Ca++ channel blocking activity was further confirmed when pre-treatment of tissue with crude extract (0.3-1 mg/ mL) caused a rightward shift in the Ca++ concentration-response curves, similar to verapamil. Loperamide also inhibited spontaneous and high K+- induced contractions and shifted the Ca++ curves to the right. These data indicate that crude extract of P. guajava possesses Ca++ antagonist-like constituent(s), which explain its inhibitory effect on gut motility.
AB - Psidium guajava L. is reputed for its medicinal use in hyperactive gut disorders. This study was aimed to investigate mechanism responsible for its medicinal use in diarrhea and gut spasm. In castor oil-induced diarrheal model, the crude extract of P. guajava (100-1,000 mg/kg), provided 20.52-81.05% protection, similar to loperamide. In isolated rabbit jejunum preparations, crude extract was found more potent against high K+ than spontaneous precontractions, similar to verapamil, with EC50 values of 0.66 (0.44-0.97; n=8) and 2.28 mg/mL (1.43-3.62; n=8), respectively, suggests calcium channel blocking activity, as a possible mode of action. The Ca++ channel blocking activity was further confirmed when pre-treatment of tissue with crude extract (0.3-1 mg/ mL) caused a rightward shift in the Ca++ concentration-response curves, similar to verapamil. Loperamide also inhibited spontaneous and high K+- induced contractions and shifted the Ca++ curves to the right. These data indicate that crude extract of P. guajava possesses Ca++ antagonist-like constituent(s), which explain its inhibitory effect on gut motility.
KW - Antidiarrheal
KW - Antispasmodic
KW - Calcium antagonist
KW - Psidium guajava
UR - http://www.scopus.com/inward/record.url?scp=84863757669&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863757669&partnerID=8YFLogxK
U2 - 10.3329/bjp.v6i2.8692
DO - 10.3329/bjp.v6i2.8692
M3 - Article
AN - SCOPUS:84863757669
SN - 1991-007X
VL - 6
SP - 100
EP - 105
JO - Bangladesh Journal of Pharmacology
JF - Bangladesh Journal of Pharmacology
IS - 2
ER -