Persistent human immunodeficiency virus-1 antigenaemia affects the expression of interleukin-7Rα on central and effector memory CD4 + and CD8+ T cell subsets

F. Mercier, M. R. Boulassel, B. Yassine-Diab, C. Tremblay, N. F. Bernard, R. P. Sekaly, J. P. Routy

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Interleukin (IL)-7 and its receptor (IL-7Rα) play important roles in regulating lymphopoiesis. Previous studies have reported that human immunodeficiency virus-1 (HIV-1) viraemia affects the expression of IL-7Rα, but its effects on CD4+ and CD8+ T cell memory subsets have not been studied. Using eight-colour flow cytometry, we compared the immunophenotypic patterns of CD4+ and CD8+ T cell subsets expressing IL-7Rα and activation markers, as well as circulating IL-7 levels, in three well-defined groups of HIV-1-infected subjects: successfully treated, viraemic and long-term non-progressor (LTNP). Compared with successfully treated and LTNP subjects, viraemic patients had reduced expression of IL-7Rα on both CD4+ and CD8+ T cells, particularly on central and effector memory T cell compartments, and substantially elevated expression of activation markers on CD8+ T cell subsets. Circulating IL-7 levels were correlated negatively with the number of CD4+ and CD8+ T cell subsets expressing IL-7Rα; these associations were stronger with CD4+ T cell subsets and mainly with central and effector memory cells. The expression of activation markers on CD4+ and CD8+ cell T subsets was not related to circulating IL-7 levels. A strong negative correlation was observed between central memory CD4+ or CD8+ T cells expressing IL-7Rα and those expressing activation markers, independently of IL-7 levels. Collectively, these results provide further insight on the role of unsuppressed viral load in disrupting the IL-7/IL-7Rα system and contributing to HIV-1 disease progression.

Original languageEnglish
Pages (from-to)72-80
Number of pages9
JournalClinical and Experimental Immunology
Volume152
Issue number1
DOIs
Publication statusPublished - Apr 2008

Keywords

  • Activation markers
  • HIV-1
  • Interleukin-7
  • Interleukin-7 receptor
  • T cell subsets

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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