Peptidomic analysis of the extensive array of host-defense peptides in skin secretions of the dodecaploid frog Xenopus ruwenzoriensis (Pipidae)

Laurent Coquet, Jolanta Kolodziejek, Thierry Jouenne, Norbert Nowotny, Jay D. King, J. Michael Conlon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Uganda clawed frog Xenopus ruwenzoriensis with a karyotype of 2n = 108 is one of the very few vertebrates with dodecaploid status. Peptidomic analysis of norepinephrine-stimulated skin secretions from this species led to the isolation and structural characterization of 23 host-defense peptides belonging to the following families: magainin (3 peptides), peptide glycine-leucine-amide (PGLa; 6 peptides), xenopsin precursor fragment (XPF; 3 peptides), caerulein precursor fragment (CPF; 8 peptides), and caerulein precursor fragment-related peptide (CPF-RP; 3 peptides). In addition, the secretions contained caerulein, identical to the peptide from Xenopus laevis, and two peptides that were identified as members of the trefoil factor family (TFF). The data indicate that silencing of the host-defense peptide genes following polyploidization has been appreciable and non-uniform. Consistent with data derived from comparison of nucleotide sequences of mitochrondrial and nuclear genes, cladistic analyses based upon the primary structures of the host-defense peptides provide support for an evolutionary scenario in which X. ruwenzoriensis arose from an allopolyploidization event involving an octoploid ancestor of the present-day frogs belonging to the Xenopus amieti species group and a tetraploid ancestor of Xenopus pygmaeus.

Original languageEnglish
Pages (from-to)18-24
Number of pages7
JournalComparative Biochemistry and Physiology - Part D: Genomics and Proteomics
Volume19
DOIs
Publication statusPublished - Sep 1 2016

Fingerprint

Pipidae
Xenopus
Anura
Skin
Peptides
Ceruletide
Magainins
Genes
Peptide Fragments
Uganda
Tetraploidy
Xenopus laevis

Keywords

  • Allopolyploidy
  • Antimicrobial peptide
  • Frog skin
  • Magainin
  • PGLa
  • Procaerulein
  • Proxenopsin
  • Xenopus

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology
  • Physiology

Cite this

Peptidomic analysis of the extensive array of host-defense peptides in skin secretions of the dodecaploid frog Xenopus ruwenzoriensis (Pipidae). / Coquet, Laurent; Kolodziejek, Jolanta; Jouenne, Thierry; Nowotny, Norbert; King, Jay D.; Conlon, J. Michael.

In: Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics, Vol. 19, 01.09.2016, p. 18-24.

Research output: Contribution to journalArticle

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