Peptide-receptive major histocompatibility complex class I molecules cycle between endoplasmic reticulum and cis-Golgi in wild-type lymphocytes

Malgorzata Garstka, Britta Borchert, Mohammed Al-Balushi, P. V K Praveen, Nicole Kühl, Irina Majoul, Rainer Duden, Sebastian Springer

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Prior to binding to a high affinity peptide and transporting it to the cell surface, major histocompatibility complex class I molecules are retained inside the cell by retention in the endoplasmic reticulum (ER), recycling through the ER-Golgi intermediate compartment and possibly the cis-Golgi, or both. Using fluorescence microscopy and a novel in vitro COPII (ER-to-ER-Golgi intermediate compartment) vesicle formation assay, we find that in both lymphocytes and fibroblasts that lack the functional transporter associated with antigen presentation, class I molecules exit the ER and reach the cis-Golgi. Intriguingly, in wild-type T1 lymphoma cells, peptide-occupied and peptide-receptive class I molecules are simultaneously exported from ER membranes with similar efficiencies. Our results suggest that binding of high affinity peptide and exit from the ER are not coupled, that the major histocompatibility complex class I quality control compartment extends into the Golgi apparatus under standard conditions, and that peptide loading onto class I molecules may occur in post-ER compartments.

Original languageEnglish
Pages (from-to)30680-30690
Number of pages11
JournalJournal of Biological Chemistry
Volume282
Issue number42
DOIs
Publication statusPublished - Oct 19 2007

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Lymphocytes
Major Histocompatibility Complex
Endoplasmic Reticulum
Peptides
Molecules
Histocompatibility Antigens Class I
Fluorescence microscopy
Fibroblasts
Quality control
Recycling
Assays
Antigen Presentation
Golgi Apparatus
Membranes
Fluorescence Microscopy
Quality Control
Lymphoma

ASJC Scopus subject areas

  • Biochemistry

Cite this

Peptide-receptive major histocompatibility complex class I molecules cycle between endoplasmic reticulum and cis-Golgi in wild-type lymphocytes. / Garstka, Malgorzata; Borchert, Britta; Al-Balushi, Mohammed; Praveen, P. V K; Kühl, Nicole; Majoul, Irina; Duden, Rainer; Springer, Sebastian.

In: Journal of Biological Chemistry, Vol. 282, No. 42, 19.10.2007, p. 30680-30690.

Research output: Contribution to journalArticle

Garstka, Malgorzata ; Borchert, Britta ; Al-Balushi, Mohammed ; Praveen, P. V K ; Kühl, Nicole ; Majoul, Irina ; Duden, Rainer ; Springer, Sebastian. / Peptide-receptive major histocompatibility complex class I molecules cycle between endoplasmic reticulum and cis-Golgi in wild-type lymphocytes. In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 42. pp. 30680-30690.
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