Pathology and viral distribution in fatal Usutu virus infections of birds from the 2001 and 2002 outbreaks in Austria

S. Chvala, J. Kolodziejek, N. Nowotny, H. Weissenböck

Research output: Contribution to journalArticle

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Abstract

In the summer of 2001, Usutu virus (USUV) was isolated for the first time in Europe, from an episode of mass mortality in Eurasian blackbirds (Turdus merula). In the present study, 40 of the birds (representing three species), confirmed as cases of USUV infection, were examined by four methods (histopathology, immunohistochemistry [IHC], in-situ hybridization [ISH] and reverse-transcriptase polymerase chain reaction [RT-PCR]). The major macroscopical finding was hepatosplenomegaly; histologically, neuronal necrosis, myocardial lesions, and coagulative necrosis of the liver and spleen were observed. IHC with cross-reactive polyclonal antibodies to West Nile virus detected viral antigen predominantly in brain neurons (40/40 birds; 100%), myocardial fibres (25/32; 78%), cells of the splenic capsule (29/33; 88%), renal glomeruli (22/35; 63%), tunica muscularis of intestines (17/22; 77%), proventricular glands (16/19; 84%), lungs (18/33; 55%) and hepatic Kupffer cells (7/38; 18%). ISH with an USUV-specific oligonucleotide probe demonstrated viral nucleic acid predominantly in brain neurons (40/40; 100%), myocardial fibres (24/33; 73%), splenic macrophages (12/34; 35%), renal tubular cells (19/36; 53%), tunica muscularis of intestines (13/32; 41%), proventricular glands (19/22; 86%), lungs (7/34; 21%) and hepatic Kupffer cells (12/38; 32%). All of 33 birds tested additionally by USUV-specific RT-PCR gave positive results.

Original languageEnglish
Pages (from-to)176-185
Number of pages10
JournalJournal of Comparative Pathology
Volume131
Issue number2-3
DOIs
Publication statusPublished - Aug 2004

Fingerprint

Usutu virus
Austria
Virus Diseases
Birds
Disease Outbreaks
Kupffer Cells
Pathology
Viruses
Reverse Transcriptase Polymerase Chain Reaction
Kupffer cells
Intestines
In Situ Hybridization
Hepatocytes
birds
Necrosis
Immunohistochemistry
infection
in situ hybridization
hepatocytes
immunohistochemistry

Keywords

  • Eurasian blackbird
  • Flavivirus
  • Usutu virus
  • Viral infection
  • West Nile virus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Pathology and viral distribution in fatal Usutu virus infections of birds from the 2001 and 2002 outbreaks in Austria. / Chvala, S.; Kolodziejek, J.; Nowotny, N.; Weissenböck, H.

In: Journal of Comparative Pathology, Vol. 131, No. 2-3, 08.2004, p. 176-185.

Research output: Contribution to journalArticle

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abstract = "In the summer of 2001, Usutu virus (USUV) was isolated for the first time in Europe, from an episode of mass mortality in Eurasian blackbirds (Turdus merula). In the present study, 40 of the birds (representing three species), confirmed as cases of USUV infection, were examined by four methods (histopathology, immunohistochemistry [IHC], in-situ hybridization [ISH] and reverse-transcriptase polymerase chain reaction [RT-PCR]). The major macroscopical finding was hepatosplenomegaly; histologically, neuronal necrosis, myocardial lesions, and coagulative necrosis of the liver and spleen were observed. IHC with cross-reactive polyclonal antibodies to West Nile virus detected viral antigen predominantly in brain neurons (40/40 birds; 100{\%}), myocardial fibres (25/32; 78{\%}), cells of the splenic capsule (29/33; 88{\%}), renal glomeruli (22/35; 63{\%}), tunica muscularis of intestines (17/22; 77{\%}), proventricular glands (16/19; 84{\%}), lungs (18/33; 55{\%}) and hepatic Kupffer cells (7/38; 18{\%}). ISH with an USUV-specific oligonucleotide probe demonstrated viral nucleic acid predominantly in brain neurons (40/40; 100{\%}), myocardial fibres (24/33; 73{\%}), splenic macrophages (12/34; 35{\%}), renal tubular cells (19/36; 53{\%}), tunica muscularis of intestines (13/32; 41{\%}), proventricular glands (19/22; 86{\%}), lungs (7/34; 21{\%}) and hepatic Kupffer cells (12/38; 32{\%}). All of 33 birds tested additionally by USUV-specific RT-PCR gave positive results.",
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