Partial characterization of proteases produced by three fungal isolates from the rhizosphere of wild yam Dioscorea wallichii

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2 Citations (Scopus)

Abstract

Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalJournal of Applied Biological Sciences
Volume3
Issue number3
Publication statusPublished - 2009

Fingerprint

Dioscorea
yam
Rhizosphere
yams
rhizosphere
Peptide Hydrolases
proteinases
enzyme
agar
immobilization
Polysorbates
Soaps
Trichoderma
Soaps (detergents)
soaps
Aspergillus
Penicillium
Enzymes
enzymes
Immobilization

Keywords

  • Destaining activity
  • Dioscorea wallichii
  • Protease
  • Zymogram

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)

Cite this

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abstract = "Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied.",
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AU - Sivakumar, Nallusamy

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AU - Saif, Al Bahry

PY - 2009

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N2 - Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied.

AB - Among the twenty different fungal members isolated from the rhizosphere of wild yam, Dioscorea wallichii, three fungal isolates were found to be the best protease producers and identified as Aspergillus sp., Penicillium sp. and Trichoderma sp. The protease production studies of these three isolates were conducted up to 12 days. After nine days there was no significant increase in the enzyme production. Maximum protease activity was observed in the range of pH 6 and temperature 30C. Protease from all the three isolates was stable up to 2 h in dettol, tween 20, tween 80 and soap solution. Among the different immobilization techniques used agar block was the most effective. The molecular weight, zymogram analysis and destaining activity of the protease enzyme were also studied.

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