Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants

Ashraf El-Kereamy, Yong Mei Bi, Kashif Mahmood, Kosala Ranathunge, Mahmoud W. Yaish, Eiji Nambara, Steven J. Rothstein

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Glutaredoxins (GRXs) are small glutathione dependent oxidoreductases that belong to the Thioredoxin (TRX) superfamily and catalyze the reduction of disulfide bonds of their substrate proteins. Plant GRXs include three different groups based on the motif sequence, namely CPYC, CGFS, and CC-type proteins. The rice CC-type proteins, OsGRX6 was identified during the screening for genes whose expression changes depending on the level of available nitrate. Overexpression of OsGRX6 in rice displayed a semi-dwarf phenotype. The OsGRX6 overexpressors contain a higher nitrogen content than the wild type, indicating that OsGRX6 plays a role in homeostatic regulation of nitrogen use. Consistent with this, OsGRX6 overexpressors displayed delayed chlorophyll degradation and senescence compared to the wild type plants. To examine if the growth defect of these transgenic lines attribute to disturbed plant hormone actions, plant hormone levels were measured. The levels of two cytokinins (CKs), 2-isopentenyladenine and trans-zeatin, and gibberellin A1 (GA1) were increased in these lines. We also found that these transgenic lines were less sensitive to exogenously applied GA, suggesting that the increase in GA1 is a result of the feedback regulation. These data suggest that OsGRX6 affects hormone signaling and nitrogen status in rice plants.

Original languageEnglish
Article number934
JournalFrontiers in Plant Science
Volume6
Issue numberNOVEMBER
DOIs
Publication statusPublished - Nov 3 2015

Fingerprint

hormones
rice
gibberellins
plant hormones
nitrogen
genetically modified organisms
isopentenyladenine
proteins
disulfide bonds
oxidoreductases
zeatin
cytokinins
nitrogen content
glutathione
nitrates
screening
chlorophyll
phenotype
gene expression
degradation

Keywords

  • CC-type
  • Cytokinin
  • GA
  • Glutaredoxin
  • Nitrogen
  • Rice

ASJC Scopus subject areas

  • Plant Science

Cite this

El-Kereamy, A., Bi, Y. M., Mahmood, K., Ranathunge, K., Yaish, M. W., Nambara, E., & Rothstein, S. J. (2015). Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants. Frontiers in Plant Science, 6(NOVEMBER), [934]. https://doi.org/10.3389/fpls.2015.00934

Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants. / El-Kereamy, Ashraf; Bi, Yong Mei; Mahmood, Kashif; Ranathunge, Kosala; Yaish, Mahmoud W.; Nambara, Eiji; Rothstein, Steven J.

In: Frontiers in Plant Science, Vol. 6, No. NOVEMBER, 934, 03.11.2015.

Research output: Contribution to journalArticle

El-Kereamy, Ashraf ; Bi, Yong Mei ; Mahmood, Kashif ; Ranathunge, Kosala ; Yaish, Mahmoud W. ; Nambara, Eiji ; Rothstein, Steven J. / Overexpression of the CC-type glutaredoxin, OsGRX6 affects hormone and nitrogen status in rice plants. In: Frontiers in Plant Science. 2015 ; Vol. 6, No. NOVEMBER.
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