Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Raju Radhajeyalakshmi; Rethinasamy Velazhahan; Ponnusamy Balasubramanian; Sabitha Doraiswamy

doi:10.1080/03235400400027382

Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Raju Radhajeyalakshmi^*, Rethinasamy Velazhahan, Ponnusamy Balasubramanian, Sabitha Doraiswamy

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.

Original language	English
Pages (from-to)	257-265
Number of pages	9
Journal	Archives of Phytopathology and Plant Protection
Volume	38
Issue number	4
DOIs	https://doi.org/10.1080/03235400400027382
Publication status	Published - Nov 2005
Externally published	Yes

Keywords

Disease resistance
Lycopersicon esculentum
Pathogenesis-related proteins
Thaumatin-like protein
Transgenic plants

ASJC Scopus subject areas

Agronomy and Crop Science

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10.1080/03235400400027382

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title = "Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani",

abstract = "A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.",

keywords = "Disease resistance, Lycopersicon esculentum, Pathogenesis-related proteins, Thaumatin-like protein, Transgenic plants",

author = "Raju Radhajeyalakshmi and Rethinasamy Velazhahan and Ponnusamy Balasubramanian and Sabitha Doraiswamy",

note = "Funding Information: This work was supported by the Council for Scientific and Industrial Research, New Delhi, India.",

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doi = "10.1080/03235400400027382",

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volume = "38",

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T1 - Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

AU - Radhajeyalakshmi, Raju

AU - Velazhahan, Rethinasamy

AU - Balasubramanian, Ponnusamy

AU - Doraiswamy, Sabitha

N1 - Funding Information: This work was supported by the Council for Scientific and Industrial Research, New Delhi, India.

PY - 2005/11

Y1 - 2005/11

N2 - A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.

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KW - Disease resistance

KW - Lycopersicon esculentum

KW - Pathogenesis-related proteins

KW - Thaumatin-like protein

KW - Transgenic plants

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Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Abstract

Keywords

ASJC Scopus subject areas

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