Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Raju Radhajeyalakshmi, Rethinasamy Velazhahan, Ponnusamy Balasubramanian, Sabitha Doraiswamy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.

Original languageEnglish
Pages (from-to)257-265
Number of pages9
JournalArchives of Phytopathology and Plant Protection
Volume38
Issue number4
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Alternaria solani
genetically modified organisms
tomatoes
proteins
Agrobacterium
blight
transgenic plants
genes
polymerase chain reaction
promoter regions
enzyme-linked immunosorbent assay
rice
pathogens

Keywords

  • Disease resistance
  • Lycopersicon esculentum
  • Pathogenesis-related proteins
  • Thaumatin-like protein
  • Transgenic plants

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani. / Radhajeyalakshmi, Raju; Velazhahan, Rethinasamy; Balasubramanian, Ponnusamy; Doraiswamy, Sabitha.

In: Archives of Phytopathology and Plant Protection, Vol. 38, No. 4, 11.2005, p. 257-265.

Research output: Contribution to journalArticle

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