Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Raju Radhajeyalakshmi; Rethinasamy Velazhahan; Ponnusamy Balasubramanian; Sabitha Doraiswamy

doi:10.1080/03235400400027382

Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Raju Radhajeyalakshmi^*, Rethinasamy Velazhahan, Ponnusamy Balasubramanian, Sabitha Doraiswamy

^*Corresponding author for this work

Crop Sciences

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.

Original language	English
Pages (from-to)	257-265
Number of pages	9
Journal	Archives of Phytopathology and Plant Protection
Volume	38
Issue number	4
DOIs	https://doi.org/10.1080/03235400400027382
Publication status	Published - Nov 2005

Keywords

Disease resistance
Lycopersicon esculentum
Pathogenesis-related proteins
Thaumatin-like protein
Transgenic plants

ASJC Scopus subject areas

Agronomy and Crop Science

Access to Document

10.1080/03235400400027382

Cite this

Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani. / Radhajeyalakshmi, Raju; Velazhahan, Rethinasamy; Balasubramanian, Ponnusamy; Doraiswamy, Sabitha.

In: Archives of Phytopathology and Plant Protection, Vol. 38, No. 4, 11.2005, p. 257-265.

Research output: Contribution to journal › Article › peer-review

@article{96ee7a617d454bf2a433359c91bae6b7,

title = "Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani",

abstract = "A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.",

keywords = "Disease resistance, Lycopersicon esculentum, Pathogenesis-related proteins, Thaumatin-like protein, Transgenic plants",

author = "Raju Radhajeyalakshmi and Rethinasamy Velazhahan and Ponnusamy Balasubramanian and Sabitha Doraiswamy",

year = "2005",

month = nov,

doi = "10.1080/03235400400027382",

language = "English",

volume = "38",

pages = "257--265",

journal = "Archives of Phytopathology and Plant Protection",

issn = "0323-5408",

publisher = "Taylor and Francis Ltd.",

number = "4",

}

TY - JOUR

T1 - Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

AU - Radhajeyalakshmi, Raju

AU - Velazhahan, Rethinasamy

AU - Balasubramanian, Ponnusamy

AU - Doraiswamy, Sabitha

PY - 2005/11

Y1 - 2005/11

N2 - A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.

AB - A cDNA encoding thaumatin-like protein (TLP) from rice was cloned into the binary vector pMON410 under the control of the CaMV 35S promoter for Agrobacterium-mediated transformation of tomato. All putative transformants were tested for the integration and expression of the chimeric gene by polymerase chain reaction (PCR) for hygromycin resistance gene (hph) and enzyme-linked immunosorbent assay (ELISA) for TLP respectively. Constitutive, high-level expression of TLP was observed in transgenic plants. The transgenic lines exhibited increased resistance to Alternaria solani, the early blight pathogen compared to non-transgenic tomato plants.

KW - Disease resistance

KW - Lycopersicon esculentum

KW - Pathogenesis-related proteins

KW - Thaumatin-like protein

KW - Transgenic plants

UR - http://www.scopus.com/inward/record.url?scp=29944446529&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29944446529&partnerID=8YFLogxK

U2 - 10.1080/03235400400027382

DO - 10.1080/03235400400027382

M3 - Article

AN - SCOPUS:29944446529

VL - 38

SP - 257

EP - 265

JO - Archives of Phytopathology and Plant Protection

JF - Archives of Phytopathology and Plant Protection

SN - 0323-5408

IS - 4

ER -

Overexpression of thaumatin-like protein in transgenic tomato plants confers enhanced resistance to Alternaria solani

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this