TY - JOUR
T1 - Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease
AU - Datta, K.
AU - Velazhahan, R.
AU - Oliva, N.
AU - Ona, I.
AU - Mew, T.
AU - Khush, G. S.
AU - Muthukrishnan, S.
AU - Datta, S. K.
PY - 1999
Y1 - 1999
N2 - A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, 'Chinsurah Boro II', 'IR72', and 'IR51500' were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
AB - A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, 'Chinsurah Boro II', 'IR72', and 'IR51500' were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.
KW - PR-5
KW - Rhizoctonia solani
KW - Rice
KW - Sheath blight disease
KW - Thaumatin-like protein
UR - http://www.scopus.com/inward/record.url?scp=0033031144&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0033031144&partnerID=8YFLogxK
U2 - 10.1007/s001220051178
DO - 10.1007/s001220051178
M3 - Article
AN - SCOPUS:0033031144
SN - 0040-5752
VL - 98
SP - 1138
EP - 1145
JO - Theoretical And Applied Genetics
JF - Theoretical And Applied Genetics
IS - 6-7
ER -