Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease

K. Datta, R. Velazhahan, N. Oliva, I. Ona, T. Mew, G. S. Khush, S. Muthukrishnan, S. K. Datta

Research output: Contribution to journalArticle

246 Citations (Scopus)

Abstract

A 1.1-kb DNA fragment containing the coding region of a thaumatin-like protein (TLP-D34), a member of the PR-5 group, was cloned into the rice transformation vector pGL2, under the control of the CaMV 35S promoter. The Indica rice cultivars, 'Chinsurah Boro II', 'IR72', and 'IR51500' were transformed with the tlp gene construct by PEG-mediated direct gene transfer to protoplasts and by biolistic transformation using immature embryos. The presence of the chimeric gene in T0, T1, and T2 transgenic plants was detected by Southern blot analysis. The presence of the expected 23-kDa TLP in transgenic plants was confirmed by Western blot analysis and by staining with Coomassie Brilliant Blue. Bioassays of transgenic plants challenged with the sheath blight pathogen, Rhizoctonia solani, indicated that over-expression of TLP resulted in enhanced resistance compared to control plants.

Original languageEnglish
Pages (from-to)1138-1145
Number of pages8
JournalTheoretical And Applied Genetics
Volume98
Issue number6-7
DOIs
Publication statusPublished - 1999

Fingerprint

Rhizoctonia
Genetically Modified Plants
Thanatephorus cucumeris
blight
transgenic plants
genetically modified organisms
rice
Biolistics
Genes
biolistics
Proteins
genes
proteins
Protoplasts
Southern Blotting
gene transfer
embryo (plant)
Biological Assay
Southern blotting
protoplasts

Keywords

  • PR-5
  • Rhizoctonia solani
  • Rice
  • Sheath blight disease
  • Thaumatin-like protein

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

Over-expression of the cloned rice thaumatin-like protein (PR-5) gene in transgenic rice plants enhances environmental friendly resistance to Rhizoctonia solani causing sheath blight disease. / Datta, K.; Velazhahan, R.; Oliva, N.; Ona, I.; Mew, T.; Khush, G. S.; Muthukrishnan, S.; Datta, S. K.

In: Theoretical And Applied Genetics, Vol. 98, No. 6-7, 1999, p. 1138-1145.

Research output: Contribution to journalArticle

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AU - Oliva, N.

AU - Ona, I.

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AU - Khush, G. S.

AU - Muthukrishnan, S.

AU - Datta, S. K.

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