Onchocerca volvulus: A Comparative Study of in Vitro Neutrophil Killing of Microfilariae and Humoral Responses in Infected and Endemic Normals

E. H. Johnson, S. Lustigman, P. H. Kass, M. Irvine, J. Browne, A. M. Prince

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Abstract

In vitro neutrophil-mediated microfilarial killing, humoral responses to crude adult worm antigens (OVAg) and to four recombinant Onchocerca antigens (GST-OV7, GST-OV103, GST-OV3.6, and MBP-OV16), as well as Western blot analysis to stage-specific Onchocerca proteins were studied in individuals from Bassa County, Liberia, infected with onchocerciasis and in endemic normals, defined as individuals without micro-filaridermia. Both groups exhibited high levels of specific serum-dependent killing of microfilariae but could not be differentiated on the basis of these results. However, infected individuals had a significantly higher frequency of antibody response to OVAg (P = 0.0001) and to GST-OV7 (P = 0.01) and were 13.3 times more likely to respond to all antigens except GST-OV103. Based on the pattern of response to the different antigens, 17 categories of specific recognition were observed. Nine of these categories were common to both the infected individuals and the endemic normals, 2 were unique to the infected individuals, and 6 were unique to the endemic normals. Among the endemic normals, we identified a subcategory of individuals who had nondetectable levels of total IgG to OVAg by ELISA and had significantly lower IgG4 responses to OVAg. These same individuals demonstrated evidence of past infection, based on serum antibodies detectable by Western blot analysis to male and female adult worms and skin microfilariae, and had a positive response to two or less of the recombinant antigens. We believe that this subcategory may represent individuals with a unique status of immunity.

Original languageEnglish
Pages (from-to)9-19
Number of pages11
JournalExperimental Parasitology
Volume81
Issue number1
DOIs
Publication statusPublished - Aug 1995

Fingerprint

Onchocerca volvulus
Microfilariae
Neutrophils
Antigens
Onchocerca
Liberia
Immunoglobulin G
Western Blotting
Onchocerciasis
Serum
Antibody Formation
Immunity
Enzyme-Linked Immunosorbent Assay
In Vitro Techniques
Skin
Antibodies
Infection
Proteins

ASJC Scopus subject areas

  • Parasitology
  • Immunology
  • Infectious Diseases

Cite this

Onchocerca volvulus : A Comparative Study of in Vitro Neutrophil Killing of Microfilariae and Humoral Responses in Infected and Endemic Normals. / Johnson, E. H.; Lustigman, S.; Kass, P. H.; Irvine, M.; Browne, J.; Prince, A. M.

In: Experimental Parasitology, Vol. 81, No. 1, 08.1995, p. 9-19.

Research output: Contribution to journalArticle

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